PMID- 16131811
OWN - NLM
STAT- MEDLINE
DCOM- 20060420
LR  - 20170118
IS  - 1660-2129 (Electronic)
IS  - 1660-2129 (Linking)
VI  - 101
IP  - 4
DP  - 2005
TI  - C-reactive protein induces NF-kappaB activation through intracellular calcium and 
      ROS in human mesangial cells.
PG  - e165-72
AB  - BACKGROUND: C-reactive protein (CRP) is known to have a direct proinflammatory 
      effect in endothelial cells. However, little is known about the effect of CRP in 
      intrinsic renal cells. We investigated the effects of CRP on the nuclear 
      factor-kappaB (NF-kappaB) activation and monocyte chemoattractant protein-1 
      (MCP-1) gene expression in human mesangial cells and also examined whether 
      intracellular calcium and reactive oxygen species (ROS) were involved in the CRP- 
      induced NF-kappaB activation. METHODS: NF-kappaB binding activity and MCP-1 mRNA 
      expression were measured by electrophoretic mobility shift assay and Northern 
      blot analysis, respectively. Intracellular calcium was monitored by confocal 
      microscopy using calcium sensitive dye, Fluo-3 and intracellular ROS production 
      was determined, using 2',7'-dichlorofluorescin diacetate. RESULTS: CRP increased 
      NF-kappaB binding activity in a dose-dependent manner (12.5-100 microg/ml), which 
      was induced within 1 h after incubation and peaked around 3 h. CRP also increased 
      the MCP-1 mRNA expression via activation of NF-kappaB. Both intracellular calcium 
      and ROS was induced by CRP. Calcium chelator, BAPTA-AM and anti-oxidants such as 
      N-acetylcysteine and tiron suppressed CRP-induced NF-kappaB activation. 
      CONCLUSION: CRP exerted a proinflammatory effect in human mesangial cells by 
      inducing MCP-1 gene expression via NF-kappaB activation, which was mediated, at 
      least in part, through intracellular calcium and ROS.
CI  - Copyright 2005 S. Karger AG, Basel.
FAU - Chang, Jai Won
AU  - Chang JW
AD  - Department of Internal Medicine, Urology, Asan Institute for Life Sciences, 
      College of Medicine, University of Ulsan, Seoul, South Korea.
FAU - Kim, Choung Soo
AU  - Kim CS
FAU - Kim, Soon Bae
AU  - Kim SB
FAU - Park, Su Kil
AU  - Park SK
FAU - Park, Jung Sik
AU  - Park JS
FAU - Lee, Sang Koo
AU  - Lee SK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050830
PL  - Switzerland
TA  - Nephron Exp Nephrol
JT  - Nephron. Experimental nephrology
JID - 101159770
RN  - 0 (Antioxidants)
RN  - 0 (Chelating Agents)
RN  - 0 (Chemokine CCL2)
RN  - 0 (NF-kappa B)
RN  - 0 (RNA, Messenger)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Recombinant Proteins)
RN  - 139890-68-9 (1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid 
      acetoxymethyl ester)
RN  - 4X87R5T106 (1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt)
RN  - 526U7A2651 (Egtazic Acid)
RN  - 9007-41-4 (C-Reactive Protein)
RN  - SY7Q814VUP (Calcium)
RN  - WYQ7N0BPYC (Acetylcysteine)
SB  - IM
MH  - 1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt/pharmacology
MH  - Acetylcysteine/pharmacology
MH  - Antioxidants/pharmacology
MH  - Blotting, Northern
MH  - C-Reactive Protein/*pharmacology/*physiology
MH  - Calcium/*metabolism
MH  - Cells, Cultured
MH  - Chelating Agents/pharmacology
MH  - Chemokine CCL2/genetics/metabolism
MH  - Dose-Response Relationship, Drug
MH  - Egtazic Acid/analogs & derivatives/pharmacology
MH  - Electrophoretic Mobility Shift Assay
MH  - Gene Expression Regulation
MH  - Humans
MH  - Inflammation
MH  - Mesangial Cells/chemistry/drug effects/*metabolism
MH  - Microscopy, Confocal
MH  - NF-kappa B/genetics/*metabolism
MH  - RNA, Messenger/analysis/genetics
MH  - Reactive Oxygen Species/*metabolism
MH  - Recombinant Proteins/pharmacology
MH  - Time Factors
EDAT- 2005/09/01 09:00
MHDA- 2006/04/21 09:00
CRDT- 2005/09/01 09:00
PHST- 2005/02/11 00:00 [received]
PHST- 2005/05/25 00:00 [accepted]
PHST- 2005/09/01 09:00 [pubmed]
PHST- 2006/04/21 09:00 [medline]
PHST- 2005/09/01 09:00 [entrez]
AID - 87940 [pii]
AID - 10.1159/000087940 [doi]
PST - ppublish
SO  - Nephron Exp Nephrol. 2005;101(4):e165-72. doi: 10.1159/000087940. Epub 2005 Aug 
      30.