PMID- 16133363
OWN - NLM
STAT- MEDLINE
DCOM- 20060207
LR  - 20181113
IS  - 0945-6317 (Print)
IS  - 0945-6317 (Linking)
VI  - 447
IP  - 6
DP  - 2005 Dec
TI  - Quantification of clonal hematopoiesis in polycythemia vera.
PG  - 947-53
AB  - Polycythemia vera (PV) is believed to represent a clonal trilineage 
      myeloaccumulative hematopoietic disorder. This study was undertaken to estimate 
      for the first time the proportion not only of the neoplastic clone but also of 
      clonal and residual nonneoplastic CD34(+) progenitor cells. Chromosomal 
      abnormalities, including trisomy 8 or 9, are phenomena associated in about 20% of 
      PV patients. Therefore, we screened peripheral blood (PB) mononuclear cells of PV 
      patients in the chronic phase of the disease and looked for chromosomal 
      abnormalities performing comparative genomic hybridization. Two of the ten 
      patients revealed cytogenetic changes, including trisomy 8 or 9. To quantify the 
      proportion of cytogenetic abnormal cells in these patients, we applied 
      fluorescence in situ hybridization (FISH) technique on immunomagnetically 
      enriched cell fractions. Ninety percent of the mononuclear cells and up to 79% of 
      PB-derived CD34(+) progenitor cells presented three signals for chromosome 8 or 
      9. The diagnostic value of FISH to detect trisomies in trephine biopsies was then 
      tested in all patients under study. Although the probability to detect FISH 
      signals in a certain section plane is reduced, constantly 10-15% of the cells 
      revealed three signals. Concerning the CD34(+) progenitor cell pool, a distinct 
      nonclonal population exists in these patients. Our data underline the stem cell 
      character of PV and additionally quantify the proportion of clonal CD34(+) 
      progenitor cells for the first time. The finding of a distinct, not aberrant, 
      CD34(+) progenitor cell population in chronic phase PV may offer perspectives in 
      treatment of the disease. Finally, FISH analysis of bone marrow biopsies can be 
      helpful to consolidate diagnosis of early PV.
FAU - Siebolts, Udo
AU  - Siebolts U
AD  - Institute of Pathology, University of Cologne, Joseph-Stelzmannstr. 9, 50924 
      Cologne, Germany.
FAU - Ates, Murat
AU  - Ates M
FAU - Spitz, Rudiger
AU  - Spitz R
FAU - Thiele, Jurgen
AU  - Thiele J
FAU - Wickenhauser, Claudia
AU  - Wickenhauser C
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050819
PL  - Germany
TA  - Virchows Arch
JT  - Virchows Archiv : an international journal of pathology
JID - 9423843
RN  - 0 (CD4 Antigens)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - CD4 Antigens/metabolism
MH  - *Chromosome Aberrations
MH  - Clone Cells
MH  - Hematopoietic Stem Cells/*pathology
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Middle Aged
MH  - *Nucleic Acid Hybridization
MH  - Polycythemia Vera/*genetics
MH  - Sensitivity and Specificity
EDAT- 2005/09/01 09:00
MHDA- 2006/02/08 09:00
CRDT- 2005/09/01 09:00
PHST- 2005/06/23 00:00 [received]
PHST- 2005/07/03 00:00 [accepted]
PHST- 2005/09/01 09:00 [pubmed]
PHST- 2006/02/08 09:00 [medline]
PHST- 2005/09/01 09:00 [entrez]
AID - 10.1007/s00428-005-0047-7 [doi]
PST - ppublish
SO  - Virchows Arch. 2005 Dec;447(6):947-53. doi: 10.1007/s00428-005-0047-7. Epub 2005 
      Aug 19.