PMID- 16135988
OWN - NLM
STAT- MEDLINE
DCOM- 20060324
LR  - 20190727
IS  - 1528-1159 (Electronic)
IS  - 0362-2436 (Linking)
VI  - 30
IP  - 17
DP  - 2005 Sep 1
TI  - Expression of transforming growth factor and basic fibroblast growth factor and 
      core protein of proteoglycan in human vertebral cartilaginous endplate of 
      adolescent idiopathic scoliosis.
PG  - 1973-8
AB  - STUDY DESIGN: To compare the expression of cytokines and core protein of 
      proteoglycan in the scoliotic concave and convex cartilaginous endplate using 
      immunohistochemical staining. OBJECTIVES: To define the possible role of 
      transforming growth factor beta 1 (TGFbeta1), basic fibroblast growth factor 
      (bFGF), and core protein of proteoglycan in the development of adolescent 
      idiopathic scoliosis. SUMMARY OF BACKGROUND DATA: Changes in the endplate 
      composition have been implicated as possible etiologic factors in the 
      pathogenesis of adolescent idiopathic scoliosis. Cytokines have exclusive effects 
      on cartilage. Thus comparing the expression of the cytokines and matrix on the 
      convex and concave sides of scoliotic endplate tissues may help to understand the 
      role of endplate tissues in the induction and/or progression of idiopathic 
      scoliosis. METHODS: The convex and concave half of cartilage endplate was 
      collected at the apex and end vertebrae from 12 patients. The expression of 
      TGFbeta1, bFGF, and core protein on both sides was examined with the 
      immunohistochemistry method, and results were analyzed with the image analysis 
      system. RESULTS: TGFbeta1, bFGF, and core protein of proteoglycan were all 
      expressed in the cytoplasm of chondrocytes in the cartilaginous endplate. The 
      area density and quantity density of TGFbeta1 and bFGF on the concave side are 
      expressed in an even significantly higher level than that on the convex side (P > 
      or = 0.05). The expression of the core protein of proteoglycan on the convex side 
      is higher than that on the concave side, the difference is not significant (P > 
      0.05). CONCLUSION: There was a significantly higher expression of TGFbeta1 and 
      bFGF, although a lower expression of the core protein on the concave side, which 
      suggests a possible etiological factor or a secondary change in the development 
      of adolescent idiopathic scoliosis.
FAU - Xu, Hongguang
AU  - Xu H
AD  - Department of Orthopedic Surgery, Peking Union Medical College Hospital, Beijing, 
      People's Republic of China. xuhg@medmail.com.cn
FAU - Qiu, Guixihg
AU  - Qiu G
FAU - Wu, Zhihong
AU  - Wu Z
FAU - Wang, Yipeng
AU  - Wang Y
FAU - Zhang, Jianguo
AU  - Zhang J
FAU - Liu, Yong
AU  - Liu Y
FAU - Yang, Xinyu
AU  - Yang X
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Spine (Phila Pa 1976)
JT  - Spine
JID - 7610646
RN  - 0 (Aggrecans)
RN  - 0 (Chondroitin Sulfate Proteoglycans)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Proteoglycans)
RN  - 0 (TGFB1 protein, human)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aggrecans
MH  - Cartilage, Articular/*metabolism/pathology
MH  - Child
MH  - Chondrocytes/metabolism
MH  - Chondroitin Sulfate Proteoglycans/*metabolism
MH  - Cytoplasm/metabolism
MH  - Extracellular Matrix Proteins/*metabolism
MH  - Female
MH  - Fibroblast Growth Factor 2/*metabolism
MH  - Humans
MH  - Immunohistochemistry/methods
MH  - Lectins, C-Type/*metabolism
MH  - Male
MH  - Proteoglycans/*metabolism
MH  - Scoliosis/*metabolism/pathology
MH  - Spine/*metabolism
MH  - Staining and Labeling
MH  - Tissue Distribution
MH  - Transforming Growth Factor beta/*metabolism
MH  - Transforming Growth Factor beta1
EDAT- 2005/09/02 09:00
MHDA- 2006/03/25 09:00
CRDT- 2005/09/02 09:00
PHST- 2005/09/02 09:00 [pubmed]
PHST- 2006/03/25 09:00 [medline]
PHST- 2005/09/02 09:00 [entrez]
AID - 00007632-200509010-00011 [pii]
AID - 10.1097/01.brs.0000176445.01967.8a [doi]
PST - ppublish
SO  - Spine (Phila Pa 1976). 2005 Sep 1;30(17):1973-8. doi: 
      10.1097/01.brs.0000176445.01967.8a.