PMID- 16136339
OWN - NLM
STAT- MEDLINE
DCOM- 20060824
LR  - 20220318
IS  - 0340-5761 (Print)
IS  - 0340-5761 (Linking)
VI  - 80
IP  - 2
DP  - 2006 Feb
TI  - Evaluation of caspase-dependent apoptosis during fluoride-induced liver lesion in 
      pigs.
PG  - 74-80
AB  - Sixteen barrows (Duroc x Landrace x Yorkshire) were randomly divided into two 
      groups, each consisting eight pigs. The groups received the same basal diet 
      supplemented with 0 and 400 mg/kg fluoride, respectively. Histological 
      examinations, including in situ terminal deoxynucleotidyl transferase-mediated 
      dUTP nick end labeling (TUNEL), Haematoxylin and Eosin staining (HE) and 
      transmission electron microscopy observation, found apoptotic hepatocytes 50 days 
      after additional 400 mg/kg fluoride treatment. The obvious DNA ladder and the 
      significantly increased both hepatic caspase-9 and caspase-3 activity indicated 
      that fluoride induced caspase-dependent apoptosis in vivo. In addition, serum 
      glutamate pyruvate transaminase (GPT) activity and hepatic lipid peroxides (LPO) 
      concentration was significantly increased. The activity of serum glutamate 
      oxaloacetate transaminase (GOT) showed an increased trend. The results suggest 
      that fluoride induces apoptosis by elevating the oxidative stress-induced lipid 
      peroxidation, causing mitochondrial dysfunction and further activating caspase-9 
      and caspase-3.
FAU - Zhan, Xiu An
AU  - Zhan XA
AD  - Feed Science Institute, College of Animal Science, Zhejiang University, No. 268, 
      Kaixuan Road, HangZhou, 310029, People's Republic of China.
FAU - Wang, Min
AU  - Wang M
FAU - Xu, Zi Rong
AU  - Xu ZR
FAU - Li, Wei Fen
AU  - Li WF
FAU - Li, Jian Xin
AU  - Li JX
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050901
PL  - Germany
TA  - Arch Toxicol
JT  - Archives of toxicology
JID - 0417615
RN  - 0 (Lipid Peroxides)
RN  - 8ZYQ1474W7 (Sodium Fluoride)
RN  - EC 2.6.1.1 (Aspartate Aminotransferases)
RN  - EC 2.6.1.2 (Alanine Transaminase)
RN  - EC 3.4.22.- (Caspase 3)
RN  - EC 3.4.22.- (Caspase 9)
RN  - EC 3.4.22.- (Caspases)
SB  - IM
MH  - Alanine Transaminase/blood
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - Aspartate Aminotransferases/blood
MH  - Caspase 3
MH  - Caspase 9
MH  - Caspases/*metabolism
MH  - Immunohistochemistry
MH  - In Situ Nick-End Labeling
MH  - Lipid Peroxides/metabolism
MH  - Liver/*drug effects/enzymology/ultrastructure
MH  - Microscopy, Electron, Transmission
MH  - Oxidative Stress/drug effects
MH  - Sodium Fluoride/*toxicity
MH  - Swine
EDAT- 2005/09/02 09:00
MHDA- 2006/08/25 09:00
CRDT- 2005/09/02 09:00
PHST- 2005/05/26 00:00 [received]
PHST- 2005/07/20 00:00 [accepted]
PHST- 2005/09/02 09:00 [pubmed]
PHST- 2006/08/25 09:00 [medline]
PHST- 2005/09/02 09:00 [entrez]
AID - 10.1007/s00204-005-0019-3 [doi]
PST - ppublish
SO  - Arch Toxicol. 2006 Feb;80(2):74-80. doi: 10.1007/s00204-005-0019-3. Epub 2005 Sep 
      1.