PMID- 16136525
OWN - NLM
STAT- MEDLINE
DCOM- 20051115
LR  - 20111117
IS  - 0173-0835 (Print)
IS  - 0173-0835 (Linking)
VI  - 26
IP  - 19
DP  - 2005 Oct
TI  - On-line chemiluminescence detection for isoelectric focusing of heme proteins on 
      microchips.
PG  - 3595-601
AB  - In this paper we present a sensitive chemiluminescence (CL) detection of heme 
      proteins coupled with microchip IEF. The detection principle was based on the 
      catalytic effects of the heme proteins on the CL reaction of luminol-H2O2 
      enhanced by para-iodophenol. The glass microchip and poly(dimethylsiloxane) 
      (PDMS)/glass microchip for IEF were fabricated using micromachining technology in 
      the laboratory. The modes of CL detection were investigated and two microchips 
      (glass, PDMS/glass) were compared. Certain proteins, such as cytochrome c, 
      myoglobin, and horseradish peroxidase, were focused by use of Pharmalyte pH 3-10 
      as ampholytes. Hydroxypropylmethylcellulose was added to the sample solution in 
      order to easily reduce protein interactions with the channel wall as well as the 
      EOF. The focused proteins were transported by salt mobilization to the CL 
      detection window. Cytochrome c, myoglobin, and horseradish peroxidase were well 
      separated within 10 min on a glass chip and the detection limits (S/N=3) were 
      1.2x10(-7), 1.6x10(-7), and 1.0x10(-10) M, respectively.
FAU - Huang, Xiangyi
AU  - Huang X
AD  - College of Chemistry and Chemical Engineering, Shanghai Jiaotong University, 
      Shanghai, P.R. China.
FAU - Ren, Jicun
AU  - Ren J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Electrophoresis
JT  - Electrophoresis
JID - 8204476
RN  - 0 (Hemeproteins)
SB  - IM
MH  - *Electrophoresis, Microchip
MH  - Glass/chemistry
MH  - Hemeproteins/*analysis
MH  - Isoelectric Focusing/*methods
MH  - Luminescent Measurements/*methods
MH  - *Online Systems
EDAT- 2005/09/02 09:00
MHDA- 2005/11/16 09:00
CRDT- 2005/09/02 09:00
PHST- 2005/09/02 09:00 [pubmed]
PHST- 2005/11/16 09:00 [medline]
PHST- 2005/09/02 09:00 [entrez]
AID - 10.1002/elps.200500076 [doi]
PST - ppublish
SO  - Electrophoresis. 2005 Oct;26(19):3595-601. doi: 10.1002/elps.200500076.