PMID- 16136729
OWN - NLM
STAT- MEDLINE
DCOM- 20051006
LR  - 20050902
IS  - 1122-9497 (Print)
IS  - 1122-9497 (Linking)
VI  - 37
IP  - 1
DP  - 2005 Apr
TI  - Necrosis in human spermatozoa. I. Ultrastructural features and FISH study in 
      semen from patients with uro-genital infections.
PG  - 67-73
AB  - Ultrastructural characteristics and meiotic segregation in spermatozoa from 
      twelve patients affected by uro-genital bacterial infections were investigated. 
      The sperm quality was examined by light and transmission electron microscopy 
      (TEM) and fluorescence in situ hybridization (FISH) analysis was performed in 
      eight out of twelve individuals in order to investigate the meiotic behaviour of 
      chromosomes namely gonosomes and chromosome 18. TEM analysis highlighted a 
      severely altered sperm morphology, typical of apoptosis and in particular, 
      necrosis. We define the ultrastructural characteristics of necrosis as involving 
      the acrosome, chromatin, mitochondrial helix, axonemal structure and plasma 
      membrane. Based on our observations, it is possible to hypothesize that infection 
      acts at the testicular level causing sperm death, due to necrosis itself or by 
      necrosis proposed as the final step of apoptosis. Moreover, FISH analysis 
      revealed the presence of altered meiotic segregation in these patients. The high 
      rate of diploidy and gonosomes disomy in our group of patients suggests the 
      possibility of a negative effect of infection and/or inflammation on sperm 
      morphogenesis.
FAU - Collodel, G
AU  - Collodel G
AD  - Department of Pediatrics, Obstetrics and Reproductive Medicine, Biology Section, 
      University of Siena, Regional Referral Center for Male Infertility, Siena 
      University General Hospital, Italy.
FAU - Baccetti, B
AU  - Baccetti B
FAU - Capitani, S
AU  - Capitani S
FAU - Moretti, E
AU  - Moretti E
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - J Submicrosc Cytol Pathol
JT  - Journal of submicroscopic cytology and pathology
JID - 8804312
SB  - IM
MH  - Adult
MH  - Aneuploidy
MH  - Apoptosis
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Infertility, Male/etiology/genetics/*pathology
MH  - Male
MH  - Microscopy, Electron, Transmission
MH  - Necrosis
MH  - Semen/*cytology
MH  - Sperm Count
MH  - Sperm Motility
MH  - Spermatozoa/physiology/*ultrastructure
MH  - Urinary Tract Infections/complications/genetics/*pathology
EDAT- 2005/09/03 09:00
MHDA- 2005/10/07 09:00
CRDT- 2005/09/03 09:00
PHST- 2005/09/03 09:00 [pubmed]
PHST- 2005/10/07 09:00 [medline]
PHST- 2005/09/03 09:00 [entrez]
PST - ppublish
SO  - J Submicrosc Cytol Pathol. 2005 Apr;37(1):67-73.