PMID- 16136732
OWN - NLM
STAT- MEDLINE
DCOM- 20051006
LR  - 20050902
IS  - 1122-9497 (Print)
IS  - 1122-9497 (Linking)
VI  - 37
IP  - 1
DP  - 2005 Apr
TI  - Necrosis in human spermatozoa. II. Ultrastructural features and FISH study in 
      semen from patients with recovered uro-genital infections.
PG  - 93-8
AB  - Inflammation of the male genital tract is a potential cause of male sterility. 
      The quality of spermatozoa from ten patients with recovered uro-genital 
      infections was examined by transmission electron microscopy (TEM); fluorescence 
      in situ hybridization (FISH) was performed on sperm nuclei in six our of ten 
      patients to investigate the frequency of aneuploidies. TEM analysis demonstrated 
      the presence of a high percentage of necrosis in all patients, whereas apoptosis 
      was present in only five of them. Meiotic segregation was altered in all analysed 
      semen samples. Recovery from infections does not seem to coincide with improved 
      sperm quality, probably because a persistent inflammatory state demonstrated by a 
      high percentage of sperm necrosis sometimes associated with the presence of white 
      blood cells (WBC) in the seminal plasma, is present. The effects of infections of 
      the male genital tract could proceed in the absence of microbial agents due to 
      immunological mechanisms involving the pattern of chemical products typical of 
      inflammation. Our results suggest that the presence of necrosis, sometimes 
      associated with apoptosis, could be considered to be an indicator of male genital 
      tract inflammation. However, further studies are necessary to test the 
      correlation between biochemical parameters and ultrastructural and molecular 
      markers of inflammation.
FAU - Moretti, E
AU  - Moretti E
AD  - Department of Pediatrics, Obstetrics and Reproductive Medicine, Biology Section, 
      University of Siena, Regional Referral Center for Male Infertility, Siena 
      University General Hospital, Italy.
FAU - Baccetti, B
AU  - Baccetti B
FAU - Capitani, S
AU  - Capitani S
FAU - Collodel, G
AU  - Collodel G
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - J Submicrosc Cytol Pathol
JT  - Journal of submicroscopic cytology and pathology
JID - 8804312
SB  - IM
MH  - Adult
MH  - Aneuploidy
MH  - Apoptosis
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Infertility, Male/etiology/genetics/*pathology
MH  - Male
MH  - Microscopy, Electron, Transmission
MH  - Necrosis
MH  - *Remission Induction
MH  - Semen/*cytology
MH  - Sperm Count
MH  - Sperm Motility
MH  - Spermatozoa/physiology/*ultrastructure
MH  - Urinary Tract Infections/complications/genetics/pathology/*therapy
EDAT- 2005/09/03 09:00
MHDA- 2005/10/07 09:00
CRDT- 2005/09/03 09:00
PHST- 2005/09/03 09:00 [pubmed]
PHST- 2005/10/07 09:00 [medline]
PHST- 2005/09/03 09:00 [entrez]
PST - ppublish
SO  - J Submicrosc Cytol Pathol. 2005 Apr;37(1):93-8.