PMID- 16137767
OWN - NLM
STAT- MEDLINE
DCOM- 20060425
LR  - 20151119
IS  - 0161-5890 (Print)
IS  - 0161-5890 (Linking)
VI  - 43
IP  - 9
DP  - 2006 Mar
TI  - The colony-stimulating factor-1 receptor is a specific marker of macrophages from 
      the bony fish gilthead seabream.
PG  - 1418-23
AB  - We report the molecular cloning of the colony-stimulating factor-1 receptor gene 
      from the bony fish gilthead seabream (sbCSF-1R). The deduced sbCSF-1R shows a 
      predicted signal sequence, a transmembrane domain and a tyrosine kinase domain, 
      all in conserved positions. A transcript showing a premature stop codon that 
      predicted the removal of 84 C-terminal amino acids was also found. RT-PCR 
      expression studies demonstrate that, although the sbCSF-1R transcripts are found 
      in different immune tissues, including gill, liver, spleen, blood, peritoneal 
      exudate, thymus and head-kidney (HK), their expression is confined to the 
      monocyte/macrophage lineage. Furthermore, the expression of sbCSF-1R might be 
      modulated by the activation stage of the macrophages, since both the infection of 
      fish and the in vitro activation of leukocytes resulted in the down-regulation of 
      gene expression. These data indicate that the CSF-1R may be used as a specific 
      probe for cells of the monocyte/macrophage lineage in the gilthead seabream, an 
      immunological tractable fish model. In addition, the functional characterisation 
      of the CSF-1R and its ligand may shed light into the mechanisms of proliferation 
      and the pathways of differentiation of macrophages in bony fish.
FAU - Roca, Francisco J
AU  - Roca FJ
AD  - Department of Cell Biology, Faculty of Biology, University of Murcia, 30100 
      Murcia, Spain.
FAU - Sepulcre, M A Pilar
AU  - Sepulcre MA
FAU - Lopez-Castejon, Gloria
AU  - Lopez-Castejon G
FAU - Meseguer, Jose
AU  - Meseguer J
FAU - Mulero, Victoriano
AU  - Mulero V
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050830
PL  - England
TA  - Mol Immunol
JT  - Molecular immunology
JID - 7905289
RN  - 0 (Biomarkers)
RN  - 0 (DNA, Complementary)
RN  - 0 (Fish Proteins)
RN  - EC 2.7.10.1 (Receptor, Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Biomarkers
MH  - Cloning, Molecular
MH  - DNA, Complementary/genetics
MH  - Fish Proteins/*genetics/immunology
MH  - Gene Expression
MH  - Leukocytes/immunology
MH  - Macrophages/*immunology
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Receptor, Macrophage Colony-Stimulating Factor/*genetics/immunology
MH  - Sea Bream/*genetics/*immunology
MH  - Sequence Homology, Amino Acid
MH  - Sequence Homology, Nucleic Acid
EDAT- 2005/09/03 09:00
MHDA- 2006/04/28 09:00
CRDT- 2005/09/03 09:00
PHST- 2005/01/18 00:00 [received]
PHST- 2005/09/03 09:00 [pubmed]
PHST- 2006/04/28 09:00 [medline]
PHST- 2005/09/03 09:00 [entrez]
AID - S0161-5890(05)00307-X [pii]
AID - 10.1016/j.molimm.2005.07.028 [doi]
PST - ppublish
SO  - Mol Immunol. 2006 Mar;43(9):1418-23. doi: 10.1016/j.molimm.2005.07.028. Epub 2005 
      Aug 30.