PMID- 1613778
OWN - NLM
STAT- MEDLINE
DCOM- 19920729
LR  - 20211216
IS  - 0022-2615 (Print)
IS  - 0022-2615 (Linking)
VI  - 36
IP  - 6
DP  - 1992 Jun
TI  - Comparison of rapid methods for detection of heat-labile (LT) and heat-stable 
      (ST) enterotoxin in Escherichia coli.
PG  - 398-402
AB  - Oxoid VET-RPLA, ST-EIA and Pharmacia Phadebact ETEC-LT enterotoxin tests were 
      compared to find a simple but reliable method for detecting enterotoxigenic 
      Escherichia coli (ETEC) in Hungary. In the Oxoid tests, all six reference LT- or 
      ST-producing strains, except one ST-producer, gave positive results. Of 11 
      reference porcine enterotoxigenic strains, all four LT-producers gave positive 
      reactions for LT but three of 10 ST-producers gave negative reactions for ST. 
      Thirteen of 50 strains from culture collections of H. Steinruck (Germany) were 
      LT+ and nine of 33 were ST+. When 31 isolates were tested simultaneously with the 
      Oxoid and the Pharmacia LT tests, 12 strains were LT+ by the Oxoid LT test but by 
      the Phadebact LT test only seven of these strains were LT+ and, of the remainder, 
      three gave uncertain results and two gave negative results. Of 69 porcine 
      strains, seven were LT+ and three ST+. Of 901 human strains isolated in Hungary, 
      10 were LT+ and one of 24 tested was ST+. In two cases, ETEC strains were 
      isolated from contacts of travellers returning from Mongolia and Bangladesh. 
      Results of comparative studies with reference strains corresponded well to those 
      of the classical toxin detection tests. The Oxoid test was rapid, sensitive, 
      specific and easy to perform and is recommended for use in screening ETEC 
      isolates.
FAU - Czirok, E
AU  - Czirok E
AD  - National Institute of Hygiene, Budapest, Hungary.
FAU - Semjen, G
AU  - Semjen G
FAU - Steinruck, H
AU  - Steinruck H
FAU - Herpay, M
AU  - Herpay M
FAU - Milch, H
AU  - Milch H
FAU - Nyomarkay, I
AU  - Nyomarkay I
FAU - Stverteczky, Z
AU  - Stverteczky Z
FAU - Szeness, A
AU  - Szeness A
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - J Med Microbiol
JT  - Journal of medical microbiology
JID - 0224131
RN  - 0 (Bacterial Toxins)
RN  - 0 (Enterotoxins)
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (heat stable toxin (E coli))
RN  - D9K3SN2LNY (heat-labile enterotoxin, E coli)
SB  - IM
MH  - Bacterial Toxins/*analysis
MH  - Enterotoxins/*analysis
MH  - Escherichia coli/*pathogenicity
MH  - *Escherichia coli Proteins
MH  - Humans
MH  - Methods
EDAT- 1992/06/01 00:00
MHDA- 1992/06/01 00:01
CRDT- 1992/06/01 00:00
PHST- 1992/06/01 00:00 [pubmed]
PHST- 1992/06/01 00:01 [medline]
PHST- 1992/06/01 00:00 [entrez]
AID - 10.1099/00222615-36-6-398 [doi]
PST - ppublish
SO  - J Med Microbiol. 1992 Jun;36(6):398-402. doi: 10.1099/00222615-36-6-398.