PMID- 16139340
OWN - NLM
STAT- MEDLINE
DCOM- 20060207
LR  - 20061115
IS  - 0065-1281 (Print)
IS  - 0065-1281 (Linking)
VI  - 107
IP  - 4
DP  - 2005
TI  - Gonadotropin stimuli increase adrenodoxin immunoreactivity in the mitochondria of 
      rat ovarian cells.
PG  - 269-77
AB  - Adrenodoxin is a component of the electron transfer system for mitochondrial 
      cytochrome P450 in steroidogenic cells. To elucidate whether the steroidogenic 
      state can modulate the amount and the distribution of adrenodoxin in the 
      mitochondria, we determined immunohistochemically the distribution of adrenodoxin 
      and 3beta-hydroxysteroid dehydrogenase (3betaHSD) in ovaries of rats under hypo- 
      and hyperfunctional states. In the ovaries of control rats, adrenodoxin was 
      distributed in the cristae of round-shaped mitochondria of both interstitial and 
      theca cells. In hypophysectomized rats, no adrenodoxin was found in the 
      mitochondria of atrophied cells. Treatment of hypophysectomized rats with human 
      chorionic gonadotropin (hCG) or Humegon restored immunostaining of adrenodoxin in 
      the interstitial and theca cells, and rendered the vesicular cristae of C- or 
      ring-shaped mitochondria surrounding the lipid droplets adrenodoxin-positive. The 
      distribution of 3betaHSD activity detected by enzyme histochemistry in these 
      ovaries matched well that of adrenodoxin immunostaining. Our results indicate 
      that immunostaining intensity of adrenodoxin in the mitochondria reflects the 
      steroidogenic state in rat ovarian cells.
FAU - Izumi, Shin-Ichi
AU  - Izumi S
AD  - Division of Oral Cytology and Cell Biology, Nagasaki University Graduate School 
      of Biomedical Sciences, 1-7-1, Sakamoto, Nagasaki 852-8588, Japan. 
      s-izumi@net.nagasaki-u.ac.jp
FAU - Mitani, Fumiko
AU  - Mitani F
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20050901
PL  - Germany
TA  - Acta Histochem
JT  - Acta histochemica
JID - 0370320
RN  - 0 (Chorionic Gonadotropin)
RN  - 12687-22-8 (Adrenodoxin)
RN  - 61489-71-2 (Menotropins)
RN  - EC 1.1.- (3-Hydroxysteroid Dehydrogenases)
SB  - IM
MH  - 3-Hydroxysteroid Dehydrogenases/*metabolism
MH  - Adrenodoxin/*metabolism
MH  - Animals
MH  - Chorionic Gonadotropin/*pharmacology
MH  - Female
MH  - Humans
MH  - Hypophysectomy
MH  - Immunohistochemistry
MH  - Menotropins/pharmacology
MH  - Microscopy, Confocal/methods
MH  - Microscopy, Electron/methods
MH  - Mitochondria/drug effects/*metabolism/ultrastructure
MH  - Ovary/*cytology/drug effects/enzymology
MH  - Rats
MH  - Rats, Wistar
EDAT- 2005/09/06 09:00
MHDA- 2006/02/08 09:00
CRDT- 2005/09/06 09:00
PHST- 2005/04/05 00:00 [received]
PHST- 2005/06/14 00:00 [revised]
PHST- 2005/06/28 00:00 [accepted]
PHST- 2005/09/06 09:00 [pubmed]
PHST- 2006/02/08 09:00 [medline]
PHST- 2005/09/06 09:00 [entrez]
AID - S0065-1281(05)00067-X [pii]
AID - 10.1016/j.acthis.2005.07.001 [doi]
PST - ppublish
SO  - Acta Histochem. 2005;107(4):269-77. doi: 10.1016/j.acthis.2005.07.001. Epub 2005 
      Sep 1.