PMID- 16139428
OWN - NLM
STAT- MEDLINE
DCOM- 20051215
LR  - 20200401
IS  - 0304-3940 (Print)
IS  - 0304-3940 (Linking)
VI  - 390
IP  - 2
DP  - 2005 Dec 23
TI  - Nucleostemin, a coordinator of self-renewal, is expressed in rat marrow stromal 
      cells and turns off after induction of neural differentiation.
PG  - 81-6
AB  - Bone marrow stromal cells (BMSCs) are pluripotent stem cells with self-renewal 
      property and potential to differentiate into a variety of cell types. 
      Identification of the genes responsible for coordination of these processes and 
      elucidation of the mechanisms underlying these events are of fundamental 
      importance. Nucleostemin, a novel p53 binding protein localized in the nucleoli 
      of ESCs, is not expressed in the differentiated cells of adult tissue, suggesting 
      a role in maintaining stem cell self-renewal. In the present study, we have 
      evaluated the expression profile of nucleostemin in rat BMSCs before and after 
      the induction of neural differentiation by RT-PCR and immunocytochemistry. The 
      profile of nucleostemin expression is then compared to the key regulators of 
      proliferation/differentiation such as: Oct-4, Nanog, Neuro D, and Cyclin D1. Our 
      data reveal that there is no detectable expression of Oct-4 and Nanog in either 
      non-differentiated or neurally differentiated BMSCs. In contrast, the expression 
      of nucleostemin is relatively high in non-differentiated BMSCs, then sharply 
      diminishes upon induction of differentiation and finally completely vanishes by 
      6h after initiation of differentiation. The disappearance of nucleostemin 
      expression coincides with the appearance of Neurofilament-M and -H, MAP2, 
      synaptophysin- and neuron-specific enolase as revealed by RT-PCR and/or 
      immunocytochemistry. Expression of Neuro D and Cyclin D1 also diminish as 
      differentiation proceeds but at much slower rates as compared to nucleostemin. In 
      conclusion, our results suggest that nucleostemin, but not Oct-4 or Nanog, is 
      expressed in BMSCs and it possibly regulates self-renewal proliferation in BMSCs.
FAU - Yaghoobi, Mohammad Mehdi
AU  - Yaghoobi MM
AD  - Department of Genetics, Faculty of Basic Sciences, Tarbiat Modarres University, 
      Tehran, PO Box 14115-175, Iran.
FAU - Mowla, Seyed Javad
AU  - Mowla SJ
FAU - Tiraihi, Taki
AU  - Tiraihi T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050901
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Biomarkers)
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Gnl3 protein, rat)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Octamer Transcription Factor-3)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
SB  - IM
MH  - Animals
MH  - Biomarkers/metabolism
MH  - Bone Marrow Cells/*cytology/metabolism
MH  - Carrier Proteins
MH  - Cell Differentiation/*physiology
MH  - Cell Shape
MH  - DNA-Binding Proteins/metabolism
MH  - GTP-Binding Proteins
MH  - Gene Expression Regulation
MH  - Homeodomain Proteins/metabolism
MH  - Neurons/cytology/*physiology
MH  - Nuclear Proteins
MH  - Octamer Transcription Factor-3/metabolism
MH  - Rats
MH  - Stromal Cells/cytology/*physiology
EDAT- 2005/09/06 09:00
MHDA- 2005/12/16 09:00
CRDT- 2005/09/06 09:00
PHST- 2005/06/01 00:00 [received]
PHST- 2005/07/20 00:00 [revised]
PHST- 2005/08/02 00:00 [accepted]
PHST- 2005/09/06 09:00 [pubmed]
PHST- 2005/12/16 09:00 [medline]
PHST- 2005/09/06 09:00 [entrez]
AID - S0304-3940(05)00887-6 [pii]
AID - 10.1016/j.neulet.2005.08.011 [doi]
PST - ppublish
SO  - Neurosci Lett. 2005 Dec 23;390(2):81-6. doi: 10.1016/j.neulet.2005.08.011. Epub 
      2005 Sep 1.