PMID- 16140404
OWN - NLM
STAT- MEDLINE
DCOM- 20051102
LR  - 20161126
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1730
IP  - 3
DP  - 2005 Sep 25
TI  - Analysis of mono-ADP-ribosyltransferase 4 gene expression in human monocytes: 
      splicing pattern and potential regulatory elements.
PG  - 173-86
AB  - Mono-ADP-ribosyltransferase (ART) 4 belongs to a family of ectoenzymes that 
      catalyze the transfer of ADP-ribose from NAD+ to a target protein. ART4 could be 
      detected on HEL cells and erythrocytes by FACS analysis while it was absent from 
      activated monocytes, despite the presence of ART4 mRNA in these cells. The 
      predicted glycosylphosphatidylinositol (GPI) linkage of ART4 could be verified by 
      showing that treatment of erythrocytes, HEL cells and ART4-transfected HEK-293-T 
      cells with phosphatidylinositol-specific phospholipase C results in a decrease in 
      ART4 expression. Furthermore, an ART4 construct carrying an Ala285Val mutation 
      that is critical for the formation of a GPI anchor failed to be expressed in 
      transfected C-33A cells. Analysis of the gene structure revealed that the first 
      of the three exons was at least 236 bp longer than previously published and that 
      splicing occurred in the coding region of the mRNA from HEL cells and monocytes. 
      When carrying out 5' inverse RACE-PCR we confirmed the existence of 5 ATGs in the 
      5' untranslated region (5'UTR). By deletion and site-directed mutagenesis of the 
      ATGs, we showed that the first two ATGs impair translation and that both the 3rd 
      and 5th ATG can be used for translation initiation after expression in C-33A 
      cells. On analysis of the 3'UTR, which contains 2 adenylate/uridylate-rich 
      elements (AREs), we detected one variant in monocytes that would be devoid of a 
      GPI-anchor signal and thus could represent a secreted form of ART4. Thus, 
      alternative splicing and the use of regulatory elements in the 5'UTR and 3'UTR 
      represent means to control ART4 expression.
FAU - Grahnert, Andreas
AU  - Grahnert A
AD  - Institute of Biology II, Dept. of Immunobiology, University of Leipzig, 
      Talstrasse 33, D-04103 Leipzig, Germany.
FAU - Friedrich, Maik
AU  - Friedrich M
FAU - Engeland, Kurt
AU  - Engeland K
FAU - Hauschildt, Sunna
AU  - Hauschildt S
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (3' Untranslated Regions)
RN  - 0 (5' Untranslated Regions)
RN  - 0 (Codon, Initiator)
RN  - 0 (Codon, Terminator)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Membrane Proteins)
RN  - 0 (RNA, Messenger)
RN  - EC 2.4.2.- (ADP Ribose Transferases)
RN  - EC 2.4.2.31 (ART4 protein, human)
RN  - HG18B9YRS7 (Valine)
RN  - I223NX31W9 (Fluorescein-5-isothiocyanate)
SB  - IM
MH  - 3' Untranslated Regions/chemistry/genetics
MH  - 5' Untranslated Regions/chemistry/genetics
MH  - ADP Ribose Transferases/*analysis/chemistry/genetics/*metabolism
MH  - *Alternative Splicing
MH  - Amino Acid Motifs
MH  - Amino Acid Sequence
MH  - Amino Acid Substitution
MH  - Base Sequence
MH  - Cell Line
MH  - Cell Line, Tumor
MH  - Cells, Cultured
MH  - Cloning, Molecular
MH  - Codon, Initiator
MH  - Codon, Terminator
MH  - Exons
MH  - Fluorescein-5-isothiocyanate
MH  - Fluorescent Antibody Technique, Indirect
MH  - Fluorescent Dyes
MH  - Gene Expression
MH  - Humans
MH  - Leukocytes, Mononuclear/*enzymology/*metabolism
MH  - Membrane Proteins/*analysis/chemistry/genetics/*metabolism
MH  - Molecular Sequence Data
MH  - RNA, Messenger/chemistry/genetics/metabolism
MH  - Regulatory Sequences, Nucleic Acid
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Homology, Amino Acid
MH  - Valine/metabolism
EDAT- 2005/09/06 09:00
MHDA- 2005/11/03 09:00
CRDT- 2005/09/06 09:00
PHST- 2005/03/17 00:00 [received]
PHST- 2005/07/29 00:00 [revised]
PHST- 2005/08/04 00:00 [accepted]
PHST- 2005/09/06 09:00 [pubmed]
PHST- 2005/11/03 09:00 [medline]
PHST- 2005/09/06 09:00 [entrez]
AID - S0167-4781(05)00204-6 [pii]
AID - 10.1016/j.bbaexp.2005.08.001 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 2005 Sep 25;1730(3):173-86. doi: 
      10.1016/j.bbaexp.2005.08.001.