PMID- 16141241
OWN - NLM
STAT- MEDLINE
DCOM- 20080805
LR  - 20050922
IS  - 0953-8178 (Print)
IS  - 0953-8178 (Linking)
VI  - 17
IP  - 10
DP  - 2005 Oct
TI  - IL-4 is more effective than IL-13 for in vitro differentiation of dendritic cells 
      from peripheral blood mononuclear cells.
PG  - 1337-46
AB  - Dendritic cells (DCs) are the most potent professional antigen-presenting cells 
      which can activate T cells to induce the primary immune response. For clinical 
      studies, DCs are often differentiated in vitro from peripheral blood mononuclear 
      cells (PBMCs) through treatment with granulocyte macrophage colony-stimulating 
      factor (GM-CSF) and IL-4. However, IL-13, a cytokine closely related to IL-4, has 
      also been reported to induce differentiation equally or more efficiently when 
      used with GM-CSF. For the present study, we compared the DC characteristics 
      exhibited by iDCs and LPS-matured DCs differentiated from PBMCs using GM-CSF and 
      IL-4 or IL-13. Physical characteristics examined include cellular morphology and 
      surface phenotype. Functional traits investigated include FITC-dextran uptake, 
      IL-10 and IL-12 production, allostimulation and cytokine production by stimulated 
      T cells and antigen-specific T cell stimulation. Compared with IL-13-derived DCs, 
      IL-4 treatment yielded more differentiated DCs, with extensive dendrites and 
      higher expression of DC-SIGN, DEC-205, CD86 and HLA-DR. In addition, IL-4 DCs 
      were more efficient at inducing allogeneic T cell proliferation and immature IL-4 
      DCs had higher endocytic activity at low FITC-dextran concentrations (1 microg 
      ml(-1)). Although IL-13 was capable of generating DCs from PBMCs, it was not as 
      effective as IL-4 in generating DC phenotype and functionality. Thus, the use of 
      GM-CSF and IL-4 is the more efficient treatment for inducing DC differentiation 
      from PBMCs.
FAU - Ahn, Justin S
AU  - Ahn JS
AD  - Department of Surgery, Faculty of Medicine and Dentistry, University of Alberta, 
      Edmonton, AB T6G 2S2, Canada.
FAU - Agrawal, Babita
AU  - Agrawal B
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050902
PL  - England
TA  - Int Immunol
JT  - International immunology
JID - 8916182
RN  - 0 (Antigens)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Interleukin-13)
RN  - 207137-56-2 (Interleukin-4)
SB  - IM
MH  - Antigens/blood
MH  - Cell Culture Techniques
MH  - Cell Differentiation/*immunology
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Dendritic Cells/*cytology/immunology
MH  - Endocytosis/immunology
MH  - HLA-DR Antigens/biosynthesis/blood
MH  - Humans
MH  - Interleukin-13/*blood
MH  - Interleukin-4/*blood
MH  - Leukocytes, Mononuclear/*cytology/immunology
MH  - T-Lymphocytes/immunology
EDAT- 2005/09/06 09:00
MHDA- 2008/08/06 09:00
CRDT- 2005/09/06 09:00
PHST- 2005/09/06 09:00 [pubmed]
PHST- 2008/08/06 09:00 [medline]
PHST- 2005/09/06 09:00 [entrez]
AID - dxh312 [pii]
AID - 10.1093/intimm/dxh312 [doi]
PST - ppublish
SO  - Int Immunol. 2005 Oct;17(10):1337-46. doi: 10.1093/intimm/dxh312. Epub 2005 Sep 
      2.