PMID- 16141434
OWN - NLM
STAT- MEDLINE
DCOM- 20060110
LR  - 20161124
IS  - 1096-6080 (Print)
IS  - 1096-0929 (Linking)
VI  - 88
IP  - 2
DP  - 2005 Dec
TI  - In utero and lactational exposure to TCDD; steroidogenic outcomes differ in male 
      and female rat pups.
PG  - 534-44
AB  - TCDD (2,3,7,8-tetrachlorodibenzo-p-dioxin) has a potency to induce decreased 
      fertility and structural reproductive anomalies in male and female mammals. While 
      the activity profile of sex steroid hormone production distinctly differs in 
      developing males and females, we wanted to analyze sex-specific effects of TCDD 
      introduced in utero and via lactation on gonadal steroidogenesis and gonadotropin 
      levels in male and female rat infant pups. One oral dose of TCDD (0, 0.04, 0.2, 
      or 1.0 microg/kg) was given to dams on gestational day (GD) 13. Plasma 
      testosterone, estradiol, progesterone, follicle stimulating hormone (FSH), 
      luteinizing hormone (LH), and gonadal mRNA levels for steroid acute regulatory 
      protein (StAR), cytochrome P-450 cholesterol side-chain cleavage (P450scc), 
      3beta-hydroxy-steroid-dehydrogenase/Delta(5)-Delta(4) isomerase type I 
      (3beta-HSD1), P-450 17alpha-hydroxylase/17,20-lyase (P450-17alpha), and 
      cytochrome P-450 aromatase (P450arom) were determined on postnatal days (PND) 
      10-16. TCDD 1.0 mug/kg reduced body weights but did not affect relative testis 
      weight or alter testicular and ovarian histology. Plasma estradiol levels in dams 
      and female pups were reduced on PND 14 and 16. Progesterone levels remained 
      unaltered, and FSH levels were increased in female pups. In males, testosterone 
      levels were elevated on PND 10. Gonadal mRNA levels for StAR and steroidogenic 
      enzymes increased during the postnatal growth. TCDD caused no changes in 
      relatively low testicular mRNA levels. However, significant reductions in StAR 
      and P450arom mRNA levels were seen in PND 14 ovaries, and P450arom activity was 
      decreased in isolated ovarian follicles. We conclude that developing testis and 
      male gonadotropin secretion are resistant to TCDD-induced toxicity. In female 
      pups, reduced estradiol, ovarian P450arom expression and enzyme activity levels, 
      and elevated FSH levels may have a role in the development of ovarian dysfunction 
      reported in TCDD-exposed females.
FAU - Myllymaki, S A
AU  - Myllymaki SA
AD  - Department of Biology, Laboratory of Animal Physiology, University of Turku, 
      Finland.
FAU - Haavisto, T E
AU  - Haavisto TE
FAU - Brokken, L J S
AU  - Brokken LJ
FAU - Viluksela, M
AU  - Viluksela M
FAU - Toppari, J
AU  - Toppari J
FAU - Paranko, J
AU  - Paranko J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050901
PL  - United States
TA  - Toxicol Sci
JT  - Toxicological sciences : an official journal of the Society of Toxicology
JID - 9805461
RN  - 0 (Enzymes)
RN  - 0 (Hormones)
RN  - 0 (Phosphoproteins)
RN  - 0 (Polychlorinated Dibenzodioxins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Teratogens)
RN  - 0 (steroidogenic acute regulatory protein)
SB  - IM
MH  - *Abnormalities, Drug-Induced
MH  - Animals
MH  - Animals, Newborn
MH  - Dose-Response Relationship, Drug
MH  - Enzymes/genetics/metabolism
MH  - Female
MH  - Gene Expression Regulation, Developmental/drug effects
MH  - Hormones/blood
MH  - Lactation/*drug effects
MH  - Male
MH  - *Maternal Exposure
MH  - Ovary/*drug effects/metabolism
MH  - Phosphoproteins/genetics/metabolism
MH  - Polychlorinated Dibenzodioxins/*toxicity
MH  - Pregnancy/blood
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Teratogens/*toxicity
MH  - Testis/*drug effects/metabolism
EDAT- 2005/09/06 09:00
MHDA- 2006/01/13 09:00
CRDT- 2005/09/06 09:00
PHST- 2005/09/06 09:00 [pubmed]
PHST- 2006/01/13 09:00 [medline]
PHST- 2005/09/06 09:00 [entrez]
AID - kfi308 [pii]
AID - 10.1093/toxsci/kfi308 [doi]
PST - ppublish
SO  - Toxicol Sci. 2005 Dec;88(2):534-44. doi: 10.1093/toxsci/kfi308. Epub 2005 Sep 1.