PMID- 16141514
OWN - NLM
STAT- MEDLINE
DCOM- 20051028
LR  - 20190720
IS  - 0918-6158 (Print)
IS  - 0918-6158 (Linking)
VI  - 28
IP  - 9
DP  - 2005 Sep
TI  - Live cell imaging to study signaling molecules in allergic reactions.
PG  - 1551-9
AB  - Mast cells are widely distributed throughout the body, predominantly near blood 
      vessels and nerves, and express effector functions in allergic reactions, 
      inflammatory diseases, and host defense. The activation of mast cells results in 
      secretion of the preformed chemical mediators in their granules by a regulated 
      process of exocytosis and leads to synthesis and secretion of lipid mediators and 
      cytokines. Their soluble factors contribute to allergic inflammation. Mast cells 
      are associated with hypersensitivity reactions, not only in the classical 
      immunoglobulin E (IgE)-dependent mechanism but also in an IgE-independent manner. 
      In particular, investigations of potential anatomical and functional interactions 
      between mast cells and the nervous system have recently attracted great interest. 
      To understand these molecular mechanisms in mast cell activation, the ability to 
      visualize, track, and quantify molecules and events in living mast cells is an 
      essential and powerful tool. Recent dramatic advances in imaging technology and 
      labeling techniques have enabled us to carry out these tasks with high 
      spatiotemporal resolution using confocal laser scanning microscopes, green 
      fluorescent protein and its derivatives, and image analysis systems. Here we 
      review our investigations of the dynamic processes of intracellular signaling 
      molecules, cellular structure, and interactions with neurons in mast cells to 
      provide basic and valuable information for allergy and clinical immunology using 
      these new imaging methods.
FAU - Furuno, Tadahide
AU  - Furuno T
AD  - Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya, 
      Japan. furuno@dpc.aichi-gakuin.ac.jp
FAU - Nakanishi, Mamoru
AU  - Nakanishi M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - Japan
TA  - Biol Pharm Bull
JT  - Biological & pharmaceutical bulletin
JID - 9311984
SB  - IM
MH  - Animals
MH  - Calcium Signaling/physiology
MH  - Cell Degranulation/physiology
MH  - Gene Expression Regulation
MH  - Humans
MH  - Hypersensitivity/*pathology/*physiopathology
MH  - Mast Cells/*physiology/ultrastructure
MH  - Signal Transduction/*physiology
RF  - 81
EDAT- 2005/09/06 09:00
MHDA- 2005/10/29 09:00
CRDT- 2005/09/06 09:00
PHST- 2005/09/06 09:00 [pubmed]
PHST- 2005/10/29 09:00 [medline]
PHST- 2005/09/06 09:00 [entrez]
AID - JST.JSTAGE/bpb/28.1551 [pii]
AID - 10.1248/bpb.28.1551 [doi]
PST - ppublish
SO  - Biol Pharm Bull. 2005 Sep;28(9):1551-9. doi: 10.1248/bpb.28.1551.