PMID- 16141688
OWN - NLM
STAT- MEDLINE
DCOM- 20051202
LR  - 20190724
IS  - 0031-6903 (Print)
IS  - 0031-6903 (Linking)
VI  - 125
IP  - 9
DP  - 2005 Sep
TI  - [Confocal laser scanning microscopy to study molecular mechanism of mast cell 
      activation].
PG  - 671-83
AB  - In the immune system, mast cells are a key cell type in the pathogenesis of 
      immunoglobulin E (IgE)-dependent hypersensitivity reactions. Engagement of the 
      high-affinity IgE receptors by multivalent antigens initiates the downstream 
      activation of signal-transducing enzymes and evokes degranulation and cytokine 
      production via an increase in the intracellular Ca2+ concentration. In addition, 
      mast cells also play a prominent role in non-IgE-mediated hypersensitivity 
      reactions. Mast cells are closely apposed to nerves in vivo and are likely to be 
      regulated functionally by nerves. However, the molecular mechanisms for mast cell 
      activation in an IgE-dependent and -independent manner have not been fully 
      clarified. Confocal laser scanning microscopy has played an essential role in 
      cell biology by allowing visualization of specific intracellular signaling 
      molecules with high spatiotemporal resolution in living cells. We have studied 
      intracellular movements of Ca2+ using a specific fluorescent probe and several 
      types of signaling molecules using derivatives of green fluorescent protein in a 
      living single mast cell using a microscopic strategy. We here describe our 
      imaging analysis of the calcium signals to the nucleus, the movement of secretory 
      granules in the degranulation process, and the nucleocytoplasmic shuttling of 
      mitogen-activated protein kinase in mast cells. Further, we demonstrate that 
      direct communication between mast cells and nerves occurs. These findings provide 
      useful information from a new perspective to understand the molecular mechanisms 
      of allergic reaction and inflammation.
FAU - Furuno, Tadahide
AU  - Furuno T
AD  - Graduate School of Pharmaceutical Sciences, Nagoya City University,Tanabe-dori, 
      Nagoya, Japan. furuno@dpc.aichi-gakuin.ac.jp
LA  - jpn
PT  - Journal Article
PT  - Review
PL  - Japan
TA  - Yakugaku Zasshi
JT  - Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan
JID - 0413613
RN  - 0 (Cadm1 protein, mouse)
RN  - 0 (Cell Adhesion Molecule-1)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Cytokines)
RN  - 0 (Immunoglobulins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Receptors, IgE)
RN  - 33507-63-0 (Substance P)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Calcium/metabolism
MH  - Calcium Signaling
MH  - Cell Adhesion Molecule-1
MH  - Cell Adhesion Molecules
MH  - Cell Degranulation
MH  - Cell Nucleus/immunology
MH  - Cytokines/biosynthesis
MH  - Cytoplasm/immunology
MH  - Humans
MH  - Hypersensitivity/immunology
MH  - Immunoglobulin E/immunology
MH  - Immunoglobulins/physiology
MH  - Mast Cells/cytology/enzymology/*immunology/*ultrastructure
MH  - Membrane Proteins/physiology
MH  - Microscopy, Confocal
MH  - Mitogen-Activated Protein Kinases/physiology
MH  - Neurites/immunology
MH  - Receptors, IgE/immunology
MH  - Signal Transduction
MH  - Substance P/physiology
RF  - 46
EDAT- 2005/09/06 09:00
MHDA- 2005/12/13 09:00
CRDT- 2005/09/06 09:00
PHST- 2005/09/06 09:00 [pubmed]
PHST- 2005/12/13 09:00 [medline]
PHST- 2005/09/06 09:00 [entrez]
AID - JST.JSTAGE/yakushi/125.671 [pii]
AID - 10.1248/yakushi.125.671 [doi]
PST - ppublish
SO  - Yakugaku Zasshi. 2005 Sep;125(9):671-83. doi: 10.1248/yakushi.125.671.