PMID- 16143265
OWN - NLM
STAT- MEDLINE
DCOM- 20060530
LR  - 20061115
IS  - 1557-3117 (Electronic)
IS  - 1053-2498 (Linking)
VI  - 24
IP  - 9
DP  - 2005 Sep
TI  - Long-term observation after simultaneous lung and intra-bone marrow-bone marrow 
      transplantation.
PG  - 1415-23
AB  - BACKGROUND: Although lung transplantation is now an established treatment for 
      end-stage lung diseases, allogeneic transplantation of parenchymal organs 
      requires immunosuppressive therapy to prevent rejection. It has been reported 
      that bone marrow transplantation (BMT) induces specific tolerance to donor 
      organs. We have recently discovered a new method for BMT, which is called 
      intra-bone marrow (IBM) BMT, in which bone marrow cells (BMCs) are injected 
      directly into the bone marrow cavity. We demonstrate that IBM-BMT can be used to 
      induce tolerance even in simultaneous lung transplantations in rats without 
      administering any immunosuppressants. METHODS: Allogeneic lung transplantation 
      was carried out from Brown Norway to Lewis rats. Simultaneously, IBM-BMT was 
      carried out. RESULTS: Transplantation of nonirradiated lung or nontreated BMCs (T 
      cell-containing BMCs) induced graft vs host disease. Therefore, the donor lung 
      was irradiated, and T cells in the BMCs were depleted by anti-CD4, anti-CD5, and 
      anti-CD8 antibodies plus anti-mouse antibody-coated magnetic beads. Lung 
      allografts with conventional (intravenous) BMT failed to induce tolerance. 
      However, the recipients treated with lung allografts plus IBM-BMT, which showed 
      either mixed chimerism or full chimerism of hematopoietic cells, did not show 
      symptoms of graft rejection or graft vs host disease, even without the use of 
      immunosuppressants. CONCLUSIONS: These results suggest that simultaneous lung 
      transplantation and IBM-BMT (but not conventional BMT) is effective in inducing 
      persistent tolerance without the use of immunosuppressants.
FAU - Kaneda, Hiroyuki
AU  - Kaneda H
AD  - First Department of Pathology, Kansai Medical University, Moriguchi City, Osaka, 
      Japan.
FAU - Adachi, Yasushi
AU  - Adachi Y
FAU - Saito, Yukihito
AU  - Saito Y
FAU - Ikebukuro, Kazuya
AU  - Ikebukuro K
FAU - Machida, Hirohisa
AU  - Machida H
FAU - Suzuki, Yasuhiro
AU  - Suzuki Y
FAU - Minamino, Keizo
AU  - Minamino K
FAU - Zhang, Yuming
AU  - Zhang Y
FAU - Iwasaki, Masayoshi
AU  - Iwasaki M
FAU - Nakano, Keiji
AU  - Nakano K
FAU - Koike, Yasushi
AU  - Koike Y
FAU - Wang, Jianfeng
AU  - Wang J
FAU - Imamura, Hiroji
AU  - Imamura H
FAU - Ikehara, Susumu
AU  - Ikehara S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Heart Lung Transplant
JT  - The Journal of heart and lung transplantation : the official publication of the 
      International Society for Heart Transplantation
JID - 9102703
SB  - IM
MH  - Animals
MH  - Bone Marrow Transplantation/*immunology/methods
MH  - Graft Rejection/immunology/*prevention & control
MH  - Graft vs Host Disease/immunology/prevention & control
MH  - Lung/physiology
MH  - Lung Transplantation/*immunology
MH  - Lymphocyte Culture Test, Mixed
MH  - Rats
MH  - Rats, Inbred ACI
MH  - Rats, Inbred Lew
MH  - Spleen/cytology/immunology
MH  - T-Lymphocytes/*immunology
MH  - Transplantation Chimera
MH  - *Transplantation Conditioning
MH  - Transplantation, Heterologous
EDAT- 2005/09/07 09:00
MHDA- 2006/05/31 09:00
CRDT- 2005/09/07 09:00
PHST- 2004/02/03 00:00 [received]
PHST- 2004/07/28 00:00 [revised]
PHST- 2004/08/15 00:00 [accepted]
PHST- 2005/09/07 09:00 [pubmed]
PHST- 2006/05/31 09:00 [medline]
PHST- 2005/09/07 09:00 [entrez]
AID - S1053-2498(04)00451-6 [pii]
AID - 10.1016/j.healun.2004.08.015 [doi]
PST - ppublish
SO  - J Heart Lung Transplant. 2005 Sep;24(9):1415-23. doi: 
      10.1016/j.healun.2004.08.015.