PMID- 16144760
OWN - NLM
STAT- MEDLINE
DCOM- 20080229
LR  - 20091016
IS  - 0927-7765 (Print)
IS  - 0927-7765 (Linking)
VI  - 45
IP  - 2
DP  - 2005 Oct 10
TI  - Functionality and stability of heparin immobilized onto poly(dimethylsiloxane).
PG  - 76-81
AB  - Poly(dimethylsiloxane) (PDMS) has become an attractive material when working in 
      the field of microfluidics, mainly because of the rapid prototyping process it 
      involves. The increased surface volume ratio in microchannels makes the 
      interaction between sample and material surface highly important, evident when 
      handling complex biological samples such as plasma or blood. This study 
      demonstrates a new grade of non-covalent heparin surface that adds efficient 
      anticoagulant property to the PDMS material. The surface modification is a simple 
      and fast one-step process performed at neutral pH, optimal when working with 
      closed microsystems. The heparin formed a uniform and functional coating on 
      hydrophobic PDMS with comparatively high level of antithrombin-binding capacity. 
      In addition, long-term studies revealed that the immobilized heparin was more or 
      less stable in the microchannels over a time of three weeks. Recalcified plasma 
      in contact with native PDMS showed complete coagulation after 1h, while no fibrin 
      formation was detected in plasma incubated on heparin-coated PDMS within the same 
      time. In conclusion, we see the heparin coating developed and evaluated in this 
      study as a tool that greatly facilitates the use of PDMS in microfluidics dealing 
      with plasma or blood samples.
FAU - Thorslund, Sara
AU  - Thorslund S
AD  - Department of Engineering Sciences, Angstrom Laboratory, Uppsala University, Box 
      534, SE-751 21 Uppsala, Sweden.
FAU - Sanchez, Javier
AU  - Sanchez J
FAU - Larsson, Rolf
AU  - Larsson R
FAU - Nikolajeff, Fredrik
AU  - Nikolajeff F
FAU - Bergquist, Jonas
AU  - Bergquist J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Colloids Surf B Biointerfaces
JT  - Colloids and surfaces. B, Biointerfaces
JID - 9315133
RN  - 0 (Antithrombins)
RN  - 0 (Biocompatible Materials)
RN  - 0 (Dimethylpolysiloxanes)
RN  - 0 (Silicones)
RN  - 63148-62-9 (baysilon)
RN  - 9001-31-4 (Fibrin)
RN  - 9005-49-6 (Heparin)
SB  - IM
MH  - Antithrombins
MH  - *Biocompatible Materials
MH  - *Dimethylpolysiloxanes
MH  - Fibrin
MH  - *Heparin
MH  - Humans
MH  - *Silicones
EDAT- 2005/09/08 09:00
MHDA- 2008/03/01 09:00
CRDT- 2005/09/08 09:00
PHST- 2005/03/24 00:00 [received]
PHST- 2005/06/28 00:00 [revised]
PHST- 2005/07/07 00:00 [accepted]
PHST- 2005/09/08 09:00 [pubmed]
PHST- 2008/03/01 09:00 [medline]
PHST- 2005/09/08 09:00 [entrez]
AID - S0927-7765(05)00211-0 [pii]
AID - 10.1016/j.colsurfb.2005.07.004 [doi]
PST - ppublish
SO  - Colloids Surf B Biointerfaces. 2005 Oct 10;45(2):76-81. doi: 
      10.1016/j.colsurfb.2005.07.004.