PMID- 16145098
OWN - NLM
STAT- MEDLINE
DCOM- 20051025
LR  - 20191210
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 43
IP  - 9
DP  - 2005 Sep
TI  - Use of rMPB70 protein and ESAT-6 peptide as antigens for comparison of the 
      enzyme-linked immunosorbent, immunochromatographic, and latex bead agglutination 
      assays for serodiagnosis of bovine tuberculosis.
PG  - 4498-506
AB  - Current assays used to detect Mycobacterium bovis infection lack accuracy, 
      especially for recently infected animals, or are impractical for rapid field 
      diagnostic applications. To overcome these limitations with serological assays, a 
      synthetic peptide derived from early secretory antigenic target 6 (ESAT6-p) and a 
      recombinant major secreted immunogenic protein (rMPB70) of M. bovis were used in 
      an enzyme-linked immunosorbent assay (EIA), an immunochromatographic assay 
      (ICGA), and a latex bead agglutination assay (LBAA). Sera from noninfected, M. 
      bovis-infected, or M. avium subsp. paratuberculosis-infected (by natural and 
      experimental routes) animals were evaluated. Receiver operating characteristic 
      analysis comparing optical density values from the EIA with results of bacterial 
      culture or skin test, the reference test, established suitable cutoff values for 
      assessing sensitivity and specificity. The EIA and LBAA, respectively, had 
      sensitivities of 98.6 and 94.8%, specificities of 98.5 and 92.6%, and kappa 
      values of 0.97 and 0.88 with ESAT6-p. The EIA, ICGA, and LBAA, respectively, had 
      sensitivities of 96.8, 83.0, and 86.7%, specificities of 90.1, 99.4, and 97.8%, 
      and kappa values of 0.87, 0.85, and 0.83 with rMPB70. Examination of serial 
      samples of sera collected from experimentally M. bovis-infected cattle and deer 
      revealed that ESAT6-p-specific responses developed early after infection whereas 
      responses to rMPB70 developed later in the course of disease. The advantage of 
      the LBAA and ICGA as initial tests for multiple species is a rapid reaction 
      obtained in 2 to 3 h by LBAA or 20 min by ICGA without species-specific secondary 
      antibodies under field conditions, thus allowing immediate segregation of suspect 
      animals for further testing before culling.
FAU - Koo, Hye Cheong
AU  - Koo HC
AD  - Department of Veterinary Microbiology and Pathology, College of Veterinary 
      Medicine, Washington State University, Pullman, 99164-7040, USA.
FAU - Park, Yong Ho
AU  - Park YH
FAU - Ahn, Jongsam
AU  - Ahn J
FAU - Waters, W Ray
AU  - Waters WR
FAU - Palmer, Mitch V
AU  - Palmer MV
FAU - Hamilton, Mary Jo
AU  - Hamilton MJ
FAU - Barrington, George
AU  - Barrington G
FAU - Mosaad, Abdelaziz A
AU  - Mosaad AA
FAU - Park, Kun Taek
AU  - Park KT
FAU - Jung, Woo Kyung
AU  - Jung WK
FAU - Hwang, In Yeong
AU  - Hwang IY
FAU - Cho, Sang-Nae
AU  - Cho SN
FAU - Shin, Sang Jae
AU  - Shin SJ
FAU - Davis, William C
AU  - Davis WC
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (Antibodies, Bacterial)
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Bacterial Proteins)
RN  - 0 (ESAT-6 protein, Mycobacterium tuberculosis)
RN  - 0 (Peptides)
RN  - 0 (Reagent Kits, Diagnostic)
RN  - 0 (Recombinant Proteins)
RN  - 123338-23-8 (MPB70 protein, Mycobacterium bovis)
SB  - IM
MH  - Animals
MH  - Antibodies, Bacterial/*blood
MH  - Antigens, Bacterial/genetics/*immunology
MH  - Bacterial Proteins/genetics/*immunology
MH  - Cattle
MH  - Chromatography/methods
MH  - Deer
MH  - Diagnosis, Differential
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Latex Fixation Tests
MH  - Mycobacterium avium subsp. paratuberculosis
MH  - Mycobacterium bovis/*immunology
MH  - Paratuberculosis/diagnosis/microbiology
MH  - Peptides/genetics/*immunology
MH  - Reagent Kits, Diagnostic
MH  - Recombinant Proteins/immunology
MH  - Sensitivity and Specificity
MH  - Serologic Tests
MH  - Tuberculosis, Bovine/*diagnosis
PMC - PMC1234133
EDAT- 2005/09/08 09:00
MHDA- 2005/10/26 09:00
PMCR- 2005/09/01
CRDT- 2005/09/08 09:00
PHST- 2005/09/08 09:00 [pubmed]
PHST- 2005/10/26 09:00 [medline]
PHST- 2005/09/08 09:00 [entrez]
PHST- 2005/09/01 00:00 [pmc-release]
AID - 43/9/4498 [pii]
AID - 0988-05 [pii]
AID - 10.1128/JCM.43.9.4498-4506.2005 [doi]
PST - ppublish
SO  - J Clin Microbiol. 2005 Sep;43(9):4498-506. doi: 10.1128/JCM.43.9.4498-4506.2005.