PMID- 16145158 OWN - NLM STAT- MEDLINE DCOM- 20051025 LR - 20191210 IS - 0095-1137 (Print) IS - 1098-660X (Electronic) IS - 0095-1137 (Linking) VI - 43 IP - 9 DP - 2005 Sep TI - Rapid identification of Staphylococcus aureus in blood cultures by a combination of fluorescence in situ hybridization using peptide nucleic acid probes and flow cytometry. PG - 4855-7 AB - Fluorescence in situ hybridization (FISH) using peptide nucleic acid probes (PNAs) allows the identification of Staphylococcus aureus from human blood culture samples. We present data revealing that the combination of PNA FISH and flow cytometry is a possible approach for the noncultural identification of staphylococci in blood cultures. FAU - Hartmann, Hanna AU - Hartmann H AD - Institut fur Medizinische Mikrobiologie und Hygiene, Tubingen, Germany. FAU - Stender, Henrik AU - Stender H FAU - Schafer, Andrea AU - Schafer A FAU - Autenrieth, Ingo B AU - Autenrieth IB FAU - Kempf, Volkhard A J AU - Kempf VA LA - eng PT - Evaluation Study PT - Journal Article PL - United States TA - J Clin Microbiol JT - Journal of clinical microbiology JID - 7505564 RN - 0 (Culture Media) RN - 0 (Nucleic Acid Probes) RN - 0 (Peptide Nucleic Acids) SB - IM MH - Bacteremia/*microbiology MH - Bacterial Typing Techniques MH - Bacteriological Techniques MH - Blood/*microbiology MH - Culture Media MH - Flow Cytometry/*methods MH - Humans MH - In Situ Hybridization, Fluorescence/*methods MH - *Nucleic Acid Probes MH - Peptide Nucleic Acids/*genetics MH - Staphylococcal Infections/microbiology MH - Staphylococcus aureus/*classification/genetics/isolation & purification MH - Time Factors PMC - PMC1234125 EDAT- 2005/09/08 09:00 MHDA- 2005/10/26 09:00 PMCR- 2005/09/01 CRDT- 2005/09/08 09:00 PHST- 2005/09/08 09:00 [pubmed] PHST- 2005/10/26 09:00 [medline] PHST- 2005/09/08 09:00 [entrez] PHST- 2005/09/01 00:00 [pmc-release] AID - 43/9/4855 [pii] AID - 0772-05 [pii] AID - 10.1128/JCM.43.9.4855-4857.2005 [doi] PST - ppublish SO - J Clin Microbiol. 2005 Sep;43(9):4855-7. doi: 10.1128/JCM.43.9.4855-4857.2005.