PMID- 16146533
OWN - NLM
STAT- MEDLINE
DCOM- 20051018
LR  - 20071115
IS  - 0902-4441 (Print)
IS  - 0902-4441 (Linking)
VI  - 75
IP  - 4
DP  - 2005 Oct
TI  - Clonally rearranged T-cell receptor beta chain genes in HTLV-I carriers with 
      abnormal, non-flower-like, lymphocytes.
PG  - 280-7
AB  - BACKGROUND: The diagnosis of Adult T-cell leukemia/lymphoma ATLL subtypes in 
      human T-lymphotropic virus type I (HTLV-I) carriers based in morphology and 
      immunophenotype of lymphocytes can be challenger. We propose that polymerase 
      chain reaction (PCR) amplification of the rearranged TCR gene in HTLV-I healthy 
      carriers would be a convenient method for establishing the nature of the 
      circulating T lymphocytes in asymptomatic HTLV-I carriers, presenting only mild 
      and inconclusive signals of deviation from normality. METHODS: Using PCR, we 
      analyzed the genetic recombination pattern of the T-cell beta-chain receptor gene 
      (TCR-beta) in order to identify clonal expansion of peripheral blood T 
      lymphocytes in 17 HTLV-I-positive healthy carriers and in nine normal 
      HTLV-I-negative blood donors. To evaluate the performance of PCR in detection of 
      clonality, we also analyzed 18 patients with post-thymic/mature T-cell 
      malignancies presenting circulating abnormal lymphocytes. RESULTS: Seven of the 
      17 HTLV-I positive individuals presented circulating abnormal lymphocytes; 
      monoclonal or oligoclonal expansion of T-cells was detected in five of the 17 
      HTLV-I-positive individuals, all of them presenting abnormal lymphocytes. Clonal 
      expansion was not detected in any of the negative controls or in any of the 12 
      remaining healthy carriers. All patients in the positive control group tested 
      positive by PCR and Southern blots. Southern blots were negative for all 17 
      healthy carriers. CONCLUSIONS: PCR amplification of segments of rearranged 
      TCR-beta is reliable for allowing early detection of small populations of clonal 
      T cells in blood samples from asymptomatic HTLV-I carriers, providing an 
      additional alert in the follow-up of carriers with abnormal circulating 
      lymphocytes.
FAU - Sales, Maria M
AU  - Sales MM
AD  - Servico de Hematologia, Divisao de Laboratorio Central, Departamento de 
      Patologia/Hospital das Clinicas, Universidade de Sao Paulo, Av. Prof. Lineu 
      Prestes 1524, 05508-900-Sao Paulo, SP, Brazil.
FAU - Bezerra, Camila N A
AU  - Bezerra CN
FAU - Hiraki, Yumi
AU  - Hiraki Y
FAU - Melo, Neusa B
AU  - Melo NB
FAU - Reboucas, Nancy A
AU  - Reboucas NA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur J Haematol
JT  - European journal of haematology
JID - 8703985
SB  - IM
MH  - Adult
MH  - Blotting, Southern
MH  - Carrier State/*diagnosis/immunology
MH  - Case-Control Studies
MH  - Cell Proliferation
MH  - Clone Cells
MH  - Female
MH  - Gene Rearrangement, beta-Chain T-Cell Antigen Receptor/*genetics
MH  - HTLV-I Infections/*diagnosis/*immunology
MH  - Humans
MH  - Leukemia-Lymphoma, Adult T-Cell/immunology/prevention & control
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction
MH  - T-Lymphocytes/immunology/*pathology
EDAT- 2005/09/09 09:00
MHDA- 2005/10/19 09:00
CRDT- 2005/09/09 09:00
PHST- 2005/09/09 09:00 [pubmed]
PHST- 2005/10/19 09:00 [medline]
PHST- 2005/09/09 09:00 [entrez]
AID - EJH506 [pii]
AID - 10.1111/j.1600-0609.2005.00506.x [doi]
PST - ppublish
SO  - Eur J Haematol. 2005 Oct;75(4):280-7. doi: 10.1111/j.1600-0609.2005.00506.x.