PMID- 16146849
OWN - NLM
STAT- MEDLINE
DCOM- 20051205
LR  - 20181201
IS  - 0925-5710 (Print)
IS  - 0925-5710 (Linking)
VI  - 82
IP  - 2
DP  - 2005 Aug
TI  - Mesenchymal stem cells in stem cell transplant recipients are damaged and remain 
      of host origin.
PG  - 152-8
AB  - The aim of this study was to investigate the expansion capacity and origin of 
      bone marrow-derived mesenchymal stem cells (MSCs) in 34 patients who received a 
      sex-mismatched stem cell transplant (SCT). Polymerase chain reaction (PCR) 
      analysis of the amelogenin gene (AMEL) was used to detect donor-derived MSCs. 
      Cultured MSCs were hybridized with fluorescence in situ hybridization (FISH) 
      probes for chromosomes X and Y to distinguish cells of donor origin from those of 
      host origin. The MSCs of 31 of the 34 patients showed confluent stroma, and the 
      MSCs from 24 of these 31 patients were successfully passaged more than 5 times 
      and were able to be used for PCR and FISH analyses. The colony-forming 
      unit-fibroblast, confluence time, and passage numbers of the MSCs and the 
      colony-forming capacity of the hematopoietic progenitor cells of the patients 
      were significantly different from those of 30 healthy control subjects. Flow 
      cytometry results showed that the proportion of CD14(+)CD45(+) cells, which are 
      regarded as monocytes/macrophages, in cultured MSCs (fifth passage) was less than 
      0.04%. PCR and FISH analyses revealed that the MSC-derived cells in all 24 
      patients were from the host. In conclusion, the expansion capacity of MSCs in 
      patients who receive an SCT is damaged, and the MSCs originate from the host.
FAU - Wang, Jing
AU  - Wang J
AD  - Peking University Institute of Hematology, Peking University People's Hospital, 
      Beijing, People's Republic of China.
FAU - Liu, Kaiyan
AU  - Liu K
FAU - Lu, Dao-Pei
AU  - Lu DP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Int J Hematol
JT  - International journal of hematology
JID - 9111627
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - *Bone Marrow Cells/pathology
MH  - Cell Survival
MH  - Female
MH  - Hematologic Neoplasms/*therapy
MH  - Humans
MH  - Male
MH  - *Mesenchymal Stem Cells/pathology
MH  - Middle Aged
MH  - *Stem Cell Transplantation
MH  - Transplantation, Homologous
EDAT- 2005/09/09 09:00
MHDA- 2005/12/13 09:00
CRDT- 2005/09/09 09:00
PHST- 2005/09/09 09:00 [pubmed]
PHST- 2005/12/13 09:00 [medline]
PHST- 2005/09/09 09:00 [entrez]
AID - 1PFGXYXQMYGNJD4J [pii]
AID - 10.1532/IJH97.A10505 [doi]
PST - ppublish
SO  - Int J Hematol. 2005 Aug;82(2):152-8. doi: 10.1532/IJH97.A10505.