PMID- 16150423
OWN - NLM
STAT- MEDLINE
DCOM- 20090126
LR  - 20181201
IS  - 1872-6240 (Electronic)
IS  - 0006-8993 (Linking)
VI  - 1058
IP  - 1-2
DP  - 2005 Oct 5
TI  - Matrix-degrading enzymes tissue plasminogen activator and matrix 
      metalloprotease-3 in the hypothalamo-neurohypophysial system.
PG  - 1-9
AB  - The hypothalamo-neurohypophysial system (HNS), synthesizing arginine vasopressin 
      (AVP) and oxytocin (OXT), is well known to show structural plasticity during 
      chronic physiological stimulation such as salt loading and lactation. In the 
      present study, we undertook in the HNS to study localization and 
      activity-dependent changes in the expression of matrix-degrading enzymes such as 
      tissue plasminogen activator (tPA) and matrix metalloprotease-3 (MMP-3). Double 
      labeling confocal microscopy demonstrated that the immunoreactivity of tPA was 
      localized at AVP-positive dendrites in the supraoptic nucleus (SON) and 
      AVP-positive terminals in the neurohypophysis (NH). The immunoreactivity of tPA 
      was also seen at astrocytic processes in the HNS. Likewise, the immunoreactivity 
      of MMP-3 was observed at AVP-positive dendrites and terminals. High magnification 
      observation further revealed punctate distribution of tPA and MMP-3 
      immunoreactivity at dendrites and terminals, suggesting that they are localized 
      at neurosecretory granules. Salt loading, known as the chronic stimulation to 
      cause the structural plasticity, increased protein and mRNA levels of tPA in the 
      SON but reduced protein levels of it in the NH. The chronic stimulation also 
      increased protein levels of urokinase plasminogen activator in the SON, but the 
      stimulation did not change protein levels of MMP-3 in the SON and NH. 
      Depolarizing agent KCl released tPA from isolated neurosecretosomes, and this 
      depolarization-dependent release was abolished by verapamil, a Ca(2+) channel 
      blocker. These results demonstrate that tPA and MMP-3 are localized mainly at 
      dendrites and terminals of AVP-expressing magnocellular neurons and tPA is 
      released in an activity-dependent manner, suggesting that matrix-degrading 
      proteases are candidate molecules to be concerned with the structural plasticity 
      in the HNS.
FAU - Miyata, Seiji
AU  - Miyata S
AD  - Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Kyoto, 
      Japan. smiyata@kit.ac.jp
FAU - Nakatani, Yoshihiro
AU  - Nakatani Y
FAU - Hayashi, Noriko
AU  - Hayashi N
FAU - Nakashima, Toshihiro
AU  - Nakashima T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050916
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (RNA, Messenger)
RN  - 113-79-1 (Arginine Vasopressin)
RN  - 660YQ98I10 (Potassium Chloride)
RN  - EC 3.4.21.68 (Tissue Plasminogen Activator)
RN  - EC 3.4.21.73 (Urokinase-Type Plasminogen Activator)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Animals
MH  - Arginine Vasopressin/metabolism
MH  - Dendrites/enzymology/ultrastructure
MH  - Hypothalamo-Hypophyseal System/cytology/*enzymology/metabolism
MH  - Immunohistochemistry
MH  - Male
MH  - Matrix Metalloproteinase 3/*metabolism
MH  - Membrane Potentials/drug effects/physiology
MH  - Microscopy, Confocal
MH  - Neuronal Plasticity/physiology
MH  - Neurons/cytology/*enzymology/metabolism
MH  - Pituitary Gland, Posterior/cytology/*enzymology/metabolism
MH  - Potassium Chloride/pharmacology
MH  - Presynaptic Terminals/enzymology/ultrastructure
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Secretory Vesicles/metabolism/ultrastructure
MH  - Supraoptic Nucleus/cytology/enzymology
MH  - Tissue Plasminogen Activator/genetics/*metabolism
MH  - Up-Regulation/physiology
MH  - Urokinase-Type Plasminogen Activator/metabolism
EDAT- 2005/09/10 09:00
MHDA- 2009/01/27 09:00
CRDT- 2005/09/10 09:00
PHST- 2005/04/13 00:00 [received]
PHST- 2005/07/08 00:00 [revised]
PHST- 2005/07/13 00:00 [accepted]
PHST- 2005/09/10 09:00 [pubmed]
PHST- 2009/01/27 09:00 [medline]
PHST- 2005/09/10 09:00 [entrez]
AID - S0006-8993(05)01059-0 [pii]
AID - 10.1016/j.brainres.2005.07.027 [doi]
PST - ppublish
SO  - Brain Res. 2005 Oct 5;1058(1-2):1-9. doi: 10.1016/j.brainres.2005.07.027. Epub 
      2005 Sep 16.