PMID- 16151148
OWN - NLM
STAT- MEDLINE
DCOM- 20051101
LR  - 20181113
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 71
IP  - 9
DP  - 2005 Sep
TI  - Abundances, identity, and growth state of actinobacteria in mountain lakes of 
      different UV transparency.
PG  - 5551-9
AB  - The occurrence, identity, and activity of microbes from the class Actinobacteria 
      was studied in the surface waters of 10 oligo- to mesotrophic mountain lakes 
      located between 913 m and 2,799 m above sea level. Oligonucleotide probes were 
      designed to distinguish between individual lineages within this group by means of 
      fluorescence in situ hybridization (FISH). Bacteria of a single phylogenetic 
      lineage (acI) represented >90% of all Actinobacteria in the studied lakes, and 
      they constituted up to 70% of the total bacterial abundances. In the subset of 
      eight lakes situated above the treeline, the community contribution of bacteria 
      from the acI lineage was significantly correlated with the ambient levels of 
      solar UV radiation (UV transparency, r(2) = 0.72; P < 0.01). Three distinct 
      genotypic subpopulations were distinguished within acI that constituted varying 
      fractions of all Actinobacteria in the different lakes. The abundance of growing 
      actinobacterial cells was estimated by FISH and immunocytochemical detection of 
      bromodeoxyuridine (BrdU) incorporation into de novo-synthesized DNA. The 
      percentages of Actinobacteria with visible DNA synthesis approximately 
      corresponded to the average percentages of BrdU-positive cells in the total 
      assemblages. Actinobacteria from different subclades of the acI lineage, 
      therefore, constituted an important autochthonous element of the aquatic 
      microbial communities in many of the studied lakes, potentially also due to their 
      higher UV resistance.
FAU - Warnecke, Falk
AU  - Warnecke F
AD  - Max-Planck-Institute for Marine Microbiology, Bremen, Germany.
FAU - Sommaruga, Ruben
AU  - Sommaruga R
FAU - Sekar, Raju
AU  - Sekar R
FAU - Hofer, Julia S
AU  - Hofer JS
FAU - Pernthaler, Jakob
AU  - Pernthaler J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (DNA, Bacterial)
RN  - 0 (Oligonucleotide Probes)
RN  - G34N38R2N1 (Bromodeoxyuridine)
RN  - GMW67QNF9C (Leucine)
SB  - IM
MH  - *Actinobacteria/classification/genetics/growth & development/radiation effects
MH  - Altitude
MH  - Bromodeoxyuridine/metabolism
MH  - DNA, Bacterial/metabolism
MH  - Fresh Water/*microbiology
MH  - Genotype
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Leucine/metabolism
MH  - Oligonucleotide Probes
MH  - *Ultraviolet Rays
PMC - PMC1214628
EDAT- 2005/09/10 09:00
MHDA- 2005/11/03 09:00
PMCR- 2005/09/01
CRDT- 2005/09/10 09:00
PHST- 2005/09/10 09:00 [pubmed]
PHST- 2005/11/03 09:00 [medline]
PHST- 2005/09/10 09:00 [entrez]
PHST- 2005/09/01 00:00 [pmc-release]
AID - 71/9/5551 [pii]
AID - 0363-05 [pii]
AID - 10.1128/AEM.71.9.5551-5559.2005 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2005 Sep;71(9):5551-9. doi: 
      10.1128/AEM.71.9.5551-5559.2005.