PMID- 16153664
OWN - NLM
STAT- MEDLINE
DCOM- 20060328
LR  - 20161124
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 594
IP  - 1-2
DP  - 2006 Feb 22
TI  - The effects of exposure to different clastogens on the pattern of chromosomal 
      aberrations detected by FISH whole chromosome painting in occupationally exposed 
      individuals.
PG  - 20-9
AB  - The pattern of chromosomal aberrations (CA) was studied by fluorescence in situ 
      hybridization (FISH) technique (whole chromosomes #1 and #4 painting) in workers 
      occupationally exposed to any of the four following conditions: acrylonitrile 
      (ACN), ethyl benzene (EB), carcinogenic polycyclic aromatic hydrocarbons 
      (c-PAHs), and irradiation in nuclear power plants (NPP), respectively. Decrease 
      in the relative frequency of translocations was observed in EB group, and an 
      increase in reciprocal translocations in ACN and NPP-exposed groups. An increase 
      in a relative number of insertions was registered under all four conditions 
      (significant at ACN, EB, c-PAHs, quasisignificant at NPP-exposed groups). 
      Significant differences in the percentage of lymphocytes with aberrations on 
      chromosome #1 (58.8+/-32.7%, versus 73.8+/-33.6% in the controls, P < 0.05), and 
      chromosome #4 (47.0+/-34.1%, versus 29.4+/-32.2%, P < 0.01) were found in workers 
      exposed to ACN. Similarly, a decrease in the proportion of cells with aberration 
      on chromosome #1 (61.0+/-24.0%, versus 73.8+/-33.6%, P < 0.05) and an increase on 
      chromosome #4 (45.6+/-24.6%, versus 29.4+/-32.2%, P < 0.05) were observed in 
      workers exposed to EB. Frequency of aberrant cells (%AB.C.) as well as genomic 
      frequency of translocations (F(G)/100) increased with age (P < 0.001). Aging also 
      increased the percentage of translocations and reciprocal translocations (P < 
      0.05), but decreased the relative number of acentric fragments (P < 0.01). 
      Smoking led to significantly increased F(G)/100 (P < 0.05), but did not affect 
      the pattern of chromosomal aberrations. Our results seem to indicate that 
      different carcinogens may induce a different pattern of chromosomal aberrations.
FAU - Beskid, O
AU  - Beskid O
AD  - Institute of Experimental Medicine AS CR and Health Institute of Central Bohemia, 
      Videnska 1083, 142 20 Prague 4, Czech Republic.
FAU - Dusek, Z
AU  - Dusek Z
FAU - Solansky, I
AU  - Solansky I
FAU - Sram, R J
AU  - Sram RJ
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050908
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (Benzene Derivatives)
RN  - 0 (Mutagens)
RN  - 0 (Polycyclic Aromatic Hydrocarbons)
RN  - L5I45M5G0O (ethylbenzene)
RN  - MP1U0D42PE (Acrylonitrile)
SB  - IM
MH  - Acrylonitrile/toxicity
MH  - Adult
MH  - Benzene Derivatives/toxicity
MH  - Cells, Cultured
MH  - Chromosome Aberrations/*chemically induced
MH  - *Chromosome Painting
MH  - Humans
MH  - Lymphocytes/drug effects/radiation effects
MH  - Male
MH  - Mutagens/*toxicity
MH  - Occupational Exposure/*adverse effects
MH  - Polycyclic Aromatic Hydrocarbons/toxicity
MH  - Radiation, Ionizing
MH  - Regression Analysis
MH  - Smoking/adverse effects
EDAT- 2005/09/13 09:00
MHDA- 2006/03/29 09:00
CRDT- 2005/09/13 09:00
PHST- 2005/02/03 00:00 [received]
PHST- 2005/07/15 00:00 [revised]
PHST- 2005/07/20 00:00 [accepted]
PHST- 2005/09/13 09:00 [pubmed]
PHST- 2006/03/29 09:00 [medline]
PHST- 2005/09/13 09:00 [entrez]
AID - S0027-5107(05)00383-0 [pii]
AID - 10.1016/j.mrfmmm.2005.07.009 [doi]
PST - ppublish
SO  - Mutat Res. 2006 Feb 22;594(1-2):20-9. doi: 10.1016/j.mrfmmm.2005.07.009. Epub 
      2005 Sep 8.