PMID- 16154123
OWN - NLM
STAT- MEDLINE
DCOM- 20060302
LR  - 20051206
IS  - 0009-8981 (Print)
IS  - 0009-8981 (Linking)
VI  - 363
IP  - 1-2
DP  - 2006 Jan
TI  - Rapid and/or high-throughput genotyping for human red blood cell, platelet and 
      leukocyte antigens, and forensic applications.
PG  - 165-76
AB  - BACKGROUND: Traditionally, transfusion medicine, platelet and human leukocyte 
      antigen (HLA) typing, and forensic medicine relied on serologic studies. METHODS: 
      In recent years, molecular testing on nucleic acids has been increasingly applied 
      to these areas. Although conventional molecular diagnostic methods such as 
      PCR-sequence-specific priming, PCR-restriction fragment-length polymorphism, 
      PCR-single-strand conformation polymorphism, sequence-based typing, and DNA 
      fingerprinting have been shown to perform well, their use is limited by long 
      turnaround times, high cost, labor-intensiveness, the need for special technical 
      skills, and/or the high risk of amplicon contamination. With advance of fast 
      and/or high-throughput methods and platforms that often combine amplification and 
      detection, a new era of molecular genotyping is emerging in these fields 
      dominated by serology for a century. As new targets, short tandem repeats, 
      mitochondrial DNA and Y-chromosome sequences were introduced for forensic 
      applications. This article reviews the current status of the application of rapid 
      and/or high-throughput genotyping methods to these areas. RESULTS: The results 
      are already promising with real-time PCR, pyrosequencing, microarrays, and mass 
      spectrometry and show high concordance rates with classic serologic and earlier 
      manual molecular diagnostic methods. Exploration of other emerging methodologies 
      will likely further enhance the diagnostic utility of molecular testing in these 
      areas.
FAU - Wu, Yan Yun
AU  - Wu YY
AD  - Department of Laboratory Medicine, Yale University School of Medicine, New Haven, 
      CT 06510-3202, USA. yan.wu@yale.edu
FAU - Csako, Gyorgy
AU  - Csako G
LA  - eng
PT  - Journal Article
PT  - Review
DEP - 20050909
PL  - Netherlands
TA  - Clin Chim Acta
JT  - Clinica chimica acta; international journal of clinical chemistry
JID - 1302422
RN  - 0 (HLA Antigens)
RN  - 0 (Nucleic Acids)
SB  - IM
MH  - Blood Platelets/*metabolism
MH  - DNA Fingerprinting/methods
MH  - Erythrocytes/*metabolism
MH  - Forensic Medicine/*methods
MH  - Genotype
MH  - HLA Antigens/genetics/*metabolism
MH  - Humans
MH  - Microarray Analysis/methods
MH  - Molecular Diagnostic Techniques/*methods/trends
MH  - Nucleic Acids/*blood
MH  - Polymerase Chain Reaction/methods
MH  - Polymorphism, Single Nucleotide
MH  - Sequence Analysis, DNA/methods
RF  - 68
EDAT- 2005/09/13 09:00
MHDA- 2006/03/03 09:00
CRDT- 2005/09/13 09:00
PHST- 2005/07/07 00:00 [received]
PHST- 2005/07/08 00:00 [revised]
PHST- 2005/07/11 00:00 [accepted]
PHST- 2005/09/13 09:00 [pubmed]
PHST- 2006/03/03 09:00 [medline]
PHST- 2005/09/13 09:00 [entrez]
AID - S0009-8981(05)00435-3 [pii]
AID - 10.1016/j.cccn.2005.07.010 [doi]
PST - ppublish
SO  - Clin Chim Acta. 2006 Jan;363(1-2):165-76. doi: 10.1016/j.cccn.2005.07.010. Epub 
      2005 Sep 9.