PMID- 16154204
OWN - NLM
STAT- MEDLINE
DCOM- 20060222
LR  - 20051115
IS  - 0165-2478 (Print)
IS  - 0165-2478 (Linking)
VI  - 102
IP  - 1
DP  - 2006 Jan 15
TI  - Proinflammatory cytokine (IL-1beta, IL-6, IL-12, IL-18 and TNF-alpha) levels in 
      sera of patients with subacute cutaneous lupus erythematosus (SCLE).
PG  - 79-82
AB  - Subacute cutaneous lupus erythematosus (SCLE) is a subset of lupus erythematosus 
      that identifies patients with clinically recognized erythematous, nonscarring 
      lesions, photosensitivity and serologic abnormalities. Anti-Ro (SS-A) antibodies 
      are considered to be a typical immunopathologic marker of SCLE. Autoimmune 
      diseases have been also characterized by the disturbances in the cytokine 
      network. The aim of this study was to compare the concentrations of 
      proinflammatory cytokines (IL-1beta, IL-6, IL-12, IL-18 and TNF-alpha) in serum 
      of ANA-positive (antibody against nuclear antigen) and ANA-negative patients with 
      SCLE. Sera samples were collected from 15 patients with SCLE (9 ANA-positive and 
      6 ANA-negative ones). The preliminary identification of autoantibodies as well as 
      their titers was determined on HEp-2 cells using IIF method. Western blotting 
      (EUROIMMUN) was applied to verify the results of IIF. Proinflammatory cytokine 
      concentrations in the patients' sera samples were determined by enzyme-linked 
      immunosorbent assay (ELISA) (Bender MedSystems). The levels of IL-12 were higher 
      in ANA-positive patients than in ANA-negative subgroups [median (interquartile 
      range), 330 pg/ml (128-708 pg/ml) versus 39.4 pg/ml (31.25-80 pg/ml)]. Similar 
      differences were observed in the level of IL-18 [median (interquartile range), 
      508.4 pg/ml (180-1222 pg/ml) versus 100.5 pg/ml (78.1-154 pg/ml)]. The 
      differences in TNF-alpha levels between the groups of ANA-positive and 
      ANA-negative patients were at the verge of statistical significance, p<0.05. The 
      sera levels of IL-1beta and IL-6 were low and of no significant difference 
      concerning the ANA-positive and ANA-negative subgroups. Since serum levels of 
      IL-12 and IL-18 were higher in ANA-positive patients than in ANA-negative 
      patients, these cytokines might play an important role in the inflammatory 
      process in SCLE.
FAU - Maczynska, Iwona
AU  - Maczynska I
AD  - Department of Microbiology and Immunology, Pomeranian Medical University, Powst. 
      Wlkp. 72, 70-111 Szczecin, Poland. iwona_ma@sci.pam.szczecin.pl
FAU - Millo, Barbara
AU  - Millo B
FAU - Ratajczak-Stefanska, Violetta
AU  - Ratajczak-Stefanska V
FAU - Maleszka, Romuald
AU  - Maleszka R
FAU - Szych, Zbigniew
AU  - Szych Z
FAU - Kurpisz, Maciej
AU  - Kurpisz M
FAU - Giedrys-Kalemba, Stefania
AU  - Giedrys-Kalemba S
LA  - eng
PT  - Journal Article
DEP - 20050825
PL  - Netherlands
TA  - Immunol Lett
JT  - Immunology letters
JID - 7910006
RN  - 0 (Cytokines)
RN  - 0 (Interleukins)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Cell Line
MH  - Cytokines/*blood
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Inflammation/blood
MH  - Interleukins/blood
MH  - Lupus Erythematosus, Cutaneous/*blood/*pathology
MH  - Tumor Necrosis Factor-alpha/analysis
EDAT- 2005/09/13 09:00
MHDA- 2006/02/24 09:00
CRDT- 2005/09/13 09:00
PHST- 2005/06/14 00:00 [received]
PHST- 2005/09/13 09:00 [pubmed]
PHST- 2006/02/24 09:00 [medline]
PHST- 2005/09/13 09:00 [entrez]
AID - S0165-2478(05)00186-0 [pii]
AID - 10.1016/j.imlet.2005.08.001 [doi]
PST - ppublish
SO  - Immunol Lett. 2006 Jan 15;102(1):79-82. doi: 10.1016/j.imlet.2005.08.001. Epub 
      2005 Aug 25.