PMID- 16154523
OWN - NLM
STAT- MEDLINE
DCOM- 20051031
LR  - 20191210
IS  - 1570-0232 (Print)
IS  - 1570-0232 (Linking)
VI  - 825
IP  - 1
DP  - 2005 Oct 15
TI  - Simultaneous enantioselective determination of amphetamine and congeners in hair 
      specimens by negative chemical ionization gas chromatography-mass spectrometry.
PG  - 57-62
AB  - Enantioselective quantification of amphetamine (AM), methamphetamine (MA), 
      3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxymethamphetamine (MDMA) and 
      3,4-methylenedioxyethylamphetamine (MDEA) enantiomers in hair using gas 
      chromatography-mass spectrometry (GC-MS) is described. Hair specimens were 
      digested with 1M sodium hydroxide at 100 degrees C for 30 min and extracted by a 
      solid phase procedure using Cleanscreen ZSDAU020. Extracted analytes were 
      derivatised with (S)-heptafluorobutyrylprolyl chloride and the resulting 
      diastereoisomers were quantified by GC-MS operating in the negative chemical 
      ionization mode. Extraction yields were between 73.0 and 97.9%. Limits of 
      detection varied in the range of 2.1-45.9 pg/mg hair, whereas the lowest limits 
      of quantification varied between 4.3 and 91.8 pg/mg hair. Intra- and inter-assay 
      precision and respective accuracy were acceptable. The enantiomeric ratios (R 
      versus S) of AM, MA, MDA, MDMA and MDEA were determined in hair from suspected 
      amphetamine abusers. Only MA and AM enantiomers were detectable in this 
      collective and the quantification data showed in most cases higher concentrations 
      of (R)-MA and (R)-AM than those of the corresponding (S)-enantiomers.
FAU - Martins, Liliane
AU  - Martins L
AD  - National Laboratory of Health, Toxicology Division, University of Luxembourg, 
      CRP-Sante, 162A, Avenue de la Faiencerie, Luxembourg L-1511, Luxembourg. 
      martins@cu.lu
FAU - Yegles, Michel
AU  - Yegles M
FAU - Chung, Heesun
AU  - Chung H
FAU - Wennig, Robert
AU  - Wennig R
LA  - eng
PT  - Journal Article
PT  - Validation Study
DEP - 20050127
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - CK833KGX7E (Amphetamine)
SB  - IM
MH  - Amphetamine/*analysis
MH  - Calibration
MH  - Gas Chromatography-Mass Spectrometry/*methods
MH  - Hair/*chemistry
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Stereoisomerism
EDAT- 2005/09/13 09:00
MHDA- 2005/11/01 09:00
CRDT- 2005/09/13 09:00
PHST- 2004/10/16 00:00 [received]
PHST- 2004/12/20 00:00 [revised]
PHST- 2004/12/24 00:00 [accepted]
PHST- 2005/09/13 09:00 [pubmed]
PHST- 2005/11/01 09:00 [medline]
PHST- 2005/09/13 09:00 [entrez]
AID - S1570-0232(05)00037-1 [pii]
AID - 10.1016/j.jchromb.2004.12.037 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Oct 15;825(1):57-62. doi: 
      10.1016/j.jchromb.2004.12.037. Epub 2005 Jan 27.