PMID- 16157046
OWN - NLM
STAT- MEDLINE
DCOM- 20051014
LR  - 20061115
IS  - 0366-6999 (Print)
IS  - 0366-6999 (Linking)
VI  - 118
IP  - 17
DP  - 2005 Sep 5
TI  - Construction of an allogenic chimeric mouse model for the study of the behaviors 
      of donor stem cells in vivo.
PG  - 1444-50
AB  - BACKGROUND: It is essential to establish an animal model for the elucidation of 
      the biological behaviors of stem cells in vivo. We constructed a chimeric animal 
      model by in utero transplantation for investigation of stem cell transplantation. 
      METHODS: This chimerism was achieved by injecting the stem cells derived from the 
      bone marrow of green fluorescence protein (GFP)-transgenic mice into fetal mice 
      at 13.5 days of gestation. Several methods such as polymerase chain reaction 
      (PCR), real-time PCR, fluorescence-assisted cell sorting (FACS) and fluorescence 
      in situ hybridization (FISH) were used for the observation of donor cells. 
      RESULTS: Under a fluorescence microscope, we observed the GFP cells of 
      donor-origin in a recipient. PCR, FACS analysis and FISH indicated chimerism at 
      various intervals. Real-time PCR indicated that some donor cells existed in 
      chimera for more than 6 months. CONCLUSIONS: Allogenic stem cells may exist in 
      recipients for a long time and this allogenic animal model provides a useful tool 
      for studying the behavior of hematopoietic stem cells and also offers an 
      effective model system for the study of stem cells.
FAU - Wang, Mo-lin
AU  - Wang ML
AD  - Shanghai Institute of Medical Genetics, Shanghai Children's Hospital, Shanghai 
      Jiao Tong University, Shanghai 200040, China.
FAU - Yan, Jing-bin
AU  - Yan JB
FAU - Xiao, Yan-ping
AU  - Xiao YP
FAU - Huang, Shu-zhen
AU  - Huang SZ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Chin Med J (Engl)
JT  - Chinese medical journal
JID - 7513795
SB  - IM
MH  - Animals
MH  - Female
MH  - Flow Cytometry
MH  - *Hematopoietic Stem Cell Transplantation
MH  - In Situ Hybridization, Fluorescence
MH  - Mice
MH  - Models, Animal
MH  - Polymerase Chain Reaction
MH  - Transplantation Chimera
MH  - Transplantation, Homologous
EDAT- 2005/09/15 09:00
MHDA- 2005/10/15 09:00
CRDT- 2005/09/15 09:00
PHST- 2005/09/15 09:00 [pubmed]
PHST- 2005/10/15 09:00 [medline]
PHST- 2005/09/15 09:00 [entrez]
PST - ppublish
SO  - Chin Med J (Engl). 2005 Sep 5;118(17):1444-50.