PMID- 16157267
OWN - NLM
STAT- MEDLINE
DCOM- 20060213
LR  - 20050913
IS  - 1386-6532 (Print)
IS  - 1386-6532 (Linking)
VI  - 34
IP  - 2
DP  - 2005 Oct
TI  - Prevalence of some herpesviruses in gingival crevicular fluid.
PG  - 147-52
AB  - BACKGROUND: The herpesviruses, ancient pathogens which have co-evoluted with 
      human, are etiologically associated with a number of diseases, from asymptomatic 
      to oncogenic and mortal diseases. It seems that some of them have also an 
      important role in the pathogenesis of human periodontal disease. OBJECTIVE: This 
      study aimed to determine the prevalence of Epstein-Barr virus (EBV), human 
      herpesvirus 6 (HHV-6), human herpesvirus 8 (HHV-8) and human cytomegalovirus 
      (HCMV) in gingival crevicular fluid (GCF) and, eventually, to find the 
      correlation between specific virus types and clinical parameters which are 
      important in periodontitis, like plaque index (PI), gingival index (GI) and 
      probing depth (PD). STUDY DESIGN: A polymerase chain reaction (PCR) and digestion 
      of PCR products with restriction endonuclease were employed to identify the 
      presence of EBV, HHV-6, HHV-8 and HCMV. RESULTS: Out of 66 samples of GCF taken 
      from the patients with periodontal disease, EBV was found in 29 (43.9%), HHV-6 in 
      16 (24.2%) and HCMV in 2 (3%) samples, while in the samples of healthy persons, 
      these viruses were not found. HHV-8 was detected neither in the patients with 
      periodontitis nor in healthy control group. More positive results were found in 
      clinical samples taken from people with higher PI and GI and in the samples taken 
      from the patients with medium PD (PD=3-6mm). In all HHV-6 positive samples, we 
      found only variant A; as for EBV positive samples, type A and type B were 
      identified and also co-infection with the two types. It seems that there is a 
      correlation between PI, PD and EBV types, but no correlation was found between 
      EBV types and GI or HHV-6 types and PI, PD, GI. CONCLUSIONS: The present findings 
      confirm some association between herpesviruses and human periodontitis.
FAU - Klemenc, P
AU  - Klemenc P
AD  - Institute of Microbiology and Immunology, Medical Faculty, Zaloska 4, 1000 
      Ljubljana, Slovenia. polona.klemenc@mf.uni-lj.si
FAU - Skaleric, U
AU  - Skaleric U
FAU - Artnik, B
AU  - Artnik B
FAU - Nograsek, P
AU  - Nograsek P
FAU - Marin, J
AU  - Marin J
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Clin Virol
JT  - Journal of clinical virology : the official publication of the Pan American 
      Society for Clinical Virology
JID - 9815671
SB  - IM
MH  - Adult
MH  - Aged
MH  - Cytomegalovirus/classification/isolation & purification
MH  - Dental Plaque Index
MH  - Gingival Crevicular Fluid/*virology
MH  - Herpesviridae/*classification/*isolation & purification
MH  - Herpesvirus 4, Human/classification/isolation & purification
MH  - Herpesvirus 6, Human/classification/isolation & purification
MH  - Herpesvirus 8, Human/classification/isolation & purification
MH  - Humans
MH  - Middle Aged
MH  - Periodontal Index
MH  - Periodontitis/pathology/*virology
MH  - Polymerase Chain Reaction
EDAT- 2005/09/15 09:00
MHDA- 2006/02/14 09:00
CRDT- 2005/09/15 09:00
PHST- 2004/10/18 00:00 [received]
PHST- 2005/01/10 00:00 [revised]
PHST- 2005/03/01 00:00 [accepted]
PHST- 2005/09/15 09:00 [pubmed]
PHST- 2006/02/14 09:00 [medline]
PHST- 2005/09/15 09:00 [entrez]
AID - S1386-6532(05)00084-3 [pii]
AID - 10.1016/j.jcv.2005.03.001 [doi]
PST - ppublish
SO  - J Clin Virol. 2005 Oct;34(2):147-52. doi: 10.1016/j.jcv.2005.03.001.