PMID- 16159160
OWN - NLM
STAT- MEDLINE
DCOM- 20051031
LR  - 20221207
IS  - 0021-8561 (Print)
IS  - 0021-8561 (Linking)
VI  - 53
IP  - 19
DP  - 2005 Sep 21
TI  - Determination of ascorbic acid and carotenoids in food commodities by liquid 
      chromatography with mass spectrometry detection.
PG  - 7371-6
AB  - Two methods, one to determine ascorbic acid and one to determine lycopene and 
      beta-carotene, in vegetables and fruits by liquid chromatography coupled with 
      mass spectrometry (LC-MS) have been established. The chromatographic separation 
      of the studied compounds and their MS parameters were optimized to improve 
      selectivity and sensitivity. In both methods, separation was carried out with two 
      coupled columns, first a C(18) and then a dC(18), using as mobile phase 70% 
      methanol (0.005% acetic acid) and 30% acetic acid 0.05% for ascorbic acid 
      determination and a mixture of methanol, tetrahydrofuran, and acetonitrile 
      (60:30:10 v/v/v) for carotenoid analysis in isocratic mode. The molecular ion was 
      selected for the quantification in selective ion monitoring (SIM) mode. Ascorbic 
      acid was detected with electrospray ionization probe (ESI) in negative mode, 
      while chemical ionization atmospheric pressure (APCI) in positive mode was used 
      for the target carotenoids. The methodology for ascorbic acid analysis is based 
      on an extraction with polytron using methanol and a mixture of methaphosphoric 
      acid and acetic acid. Extraction of the carotenoids was carried out with 
      tetrahydrofuran/methanol (1:1) (v/v). The proposed methods were applied, after 
      their corresponding validations, to the analysis of four varieties of tomatoes, 
      tomato in tin enriched and dried tomato, and to the analysis of mango and kiwi 
      fruits, to compare the content in these compounds. Moreover, the influence of the 
      process of freezing and the effect that the manipulation/preservation has in the 
      content of ascorbic acid in tomato have also been studied.
FAU - Frenich, A Garrido
AU  - Frenich AG
AD  - Department of Hydrogeology and Analytical Chemistry, University of Almeria, 
      Spain. agarrido@ual.es
FAU - Torres, M E Hernandez
AU  - Torres ME
FAU - Vega, A Belmonte
AU  - Vega AB
FAU - Vidal, J L Martinez
AU  - Vidal JL
FAU - Bolanos, P Plaza
AU  - Bolanos PP
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 01YAE03M7J (beta Carotene)
RN  - 36-88-4 (Carotenoids)
RN  - PQ6CK8PD0R (Ascorbic Acid)
RN  - SB0N2N0WV6 (Lycopene)
SB  - IM
MH  - Actinidia/chemistry
MH  - Ascorbic Acid/*analysis
MH  - Carotenoids/*analysis
MH  - Chromatography, Liquid/*methods
MH  - Food Analysis/*methods
MH  - Fruit/chemistry
MH  - Lycopene
MH  - Solanum lycopersicum/chemistry
MH  - Mangifera/chemistry
MH  - Mass Spectrometry/*methods
MH  - Spectrometry, Mass, Electrospray Ionization
MH  - beta Carotene/analysis
EDAT- 2005/09/15 09:00
MHDA- 2005/11/01 09:00
CRDT- 2005/09/15 09:00
PHST- 2005/09/15 09:00 [pubmed]
PHST- 2005/11/01 09:00 [medline]
PHST- 2005/09/15 09:00 [entrez]
AID - 10.1021/jf050973o [doi]
PST - ppublish
SO  - J Agric Food Chem. 2005 Sep 21;53(19):7371-6. doi: 10.1021/jf050973o.