PMID- 16159338
OWN - NLM
STAT- MEDLINE
DCOM- 20051201
LR  - 20220129
IS  - 0028-646X (Print)
IS  - 0028-646X (Linking)
VI  - 168
IP  - 1
DP  - 2005 Oct
TI  - Three-dimensional modelling of wheat endosperm development.
PG  - 253-62
AB  - We have developed methods, based on confocal microscopy and three-dimensional 
      (3D) modelling, for the analysis of complex tissues and individual nuclei. These 
      methods were used to study the development of early wheat (Triticum aestivum) 
      endosperm as a whole and of endosperm nuclei undergoing polyploidization. Fixed 
      sections of immature caryopses were either stained with SYTOX Green or used for 
      fluorescence in situ hybridization (FISH) to visualize centromeres, telomeres and 
      a rye chromosome arm substitution. Each section was imaged as a confocal image 
      stack. By using Amira 3.0 for computer image processing, rendered models were 
      produced of the whole endosperm and of individual nuclei. We followed endosperm 
      development up to the formation of a complete syncytium, which develops via a 
      dorsal and a ventral plate of nuclei in the central cell. Modelling of nuclei 
      showed that wheat chromosomes are not anchored to the nuclear membrane and become 
      more randomly positioned in endoreduplicated nuclei. This analysis produced a 
      precise description of the positioning of nuclei throughout the developing 
      endosperm and of chromosomal domains in single nuclei.
FAU - Wegel, Eva
AU  - Wegel E
AD  - Department of Cell and Developmental Biology, John Innes Centre, Norwich Research 
      Park, Colney Lane, Norwich NR4 7UH, UK.
FAU - Pilling, Emma
AU  - Pilling E
FAU - Calder, Grant
AU  - Calder G
FAU - Drea, Sinead
AU  - Drea S
FAU - Doonan, John
AU  - Doonan J
FAU - Dolan, Liam
AU  - Dolan L
FAU - Shaw, Peter
AU  - Shaw P
LA  - eng
GR  - BBS/E/J/00000129/BB_/Biotechnology and Biological Sciences Research 
      Council/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - New Phytol
JT  - The New phytologist
JID - 9882884
SB  - IM
MH  - Chromosomes, Plant/physiology
MH  - Microscopy, Confocal
MH  - Models, Structural
MH  - Ploidies
MH  - Seeds/*growth & development
MH  - Triticum/*cytology
EDAT- 2005/09/15 09:00
MHDA- 2005/12/13 09:00
CRDT- 2005/09/15 09:00
PHST- 2005/09/15 09:00 [pubmed]
PHST- 2005/12/13 09:00 [medline]
PHST- 2005/09/15 09:00 [entrez]
AID - NPH1503 [pii]
AID - 10.1111/j.1469-8137.2005.01503.x [doi]
PST - ppublish
SO  - New Phytol. 2005 Oct;168(1):253-62. doi: 10.1111/j.1469-8137.2005.01503.x.