PMID- 16159907
OWN - NLM
STAT- MEDLINE
DCOM- 20060227
LR  - 20161124
IS  - 0193-1849 (Print)
IS  - 0193-1849 (Linking)
VI  - 290
IP  - 1
DP  - 2006 Jan
TI  - Are tyrosine kinases involved in mediating contraction-stimulated muscle glucose 
      transport?
PG  - E123-E128
AB  - Muscle contractions and insulin stimulate glucose transport into muscle by 
      separate pathways. The contraction-mediated increase in glucose transport is 
      mediated by two mechanisms, one involves the activation of 5'-AMP-activated 
      protein kinase (AMPK) and the other involves the activation of 
      calcium/calmodulin-dependent protein kinase II (CAMKII). The steps leading from 
      the activation of AMPK and CAMKII to the translocation of GLUT4 to the cell 
      surface have not been identified. Studies with the use of the tyrosine kinase 
      inhibitor genistein suggest that one or more tyrosine kinases could be involved 
      in contraction-stimulated glucose transport. The purpose of the present study was 
      to determine the involvement of tyrosine kinases in contraction-stimulated 
      glucose transport in rat soleus and epitrochlearis muscles. 
      Contraction-stimulated glucose transport was completely prevented by pretreatment 
      with genistein (100 microM) and the related compound butein (100 microM). 
      However, the structurally distinct tyrosine kinase inhibitors 
      4-amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolo[3,4-d]pyridine and herbimycin did 
      not reduce contraction-stimulated glucose transport. Furthermore, genistein and 
      butein inhibited glucose transport even when muscles were exposed to these 
      compounds after being stimulated to contract. Muscle contractions did not result 
      in increases in tyrosine phosphorylation of proteins such as proline-rich 
      tyrosine kinase and SRC. These results provide evidence that tyrosine kinases do 
      not mediate contraction-stimulated glucose transport and that the inhibitory 
      effects of genistein on glucose transport result from direct inhibition of the 
      glucose transporters at the cell surface.
FAU - Wright, David C
AU  - Wright DC
AD  - Division of Geriatrics and Nutritional Sciences, Department of Medicine, 
      Washington University School of Medicine, St. Louis, MO 63110, USA.
FAU - Geiger, Paige C
AU  - Geiger PC
FAU - Han, Dong-Ho
AU  - Han DH
FAU - Holloszy, John O
AU  - Holloszy JO
LA  - eng
GR  - AG-00078/AG/NIA NIH HHS/United States
GR  - DK-18986/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20050913
PL  - United States
TA  - Am J Physiol Endocrinol Metab
JT  - American journal of physiology. Endocrinology and metabolism
JID - 100901226
RN  - 0 (AG 1879)
RN  - 0 (Benzoquinones)
RN  - 0 (Chalcones)
RN  - 0 (Glucose Transporter Type 4)
RN  - 0 (Insulin)
RN  - 0 (Lactams, Macrocyclic)
RN  - 0 (Pyrimidines)
RN  - 0 (Quinones)
RN  - 1W306TDA6S (Rifabutin)
RN  - 4WVS5M0LGF (butein)
RN  - 5S5A2Q39HX (Chalcone)
RN  - 70563-58-5 (herbimycin)
RN  - 9G2MP84A8W (Deoxyglucose)
RN  - DH2M523P0H (Genistein)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.10.2 (Focal Adhesion Kinase 2)
RN  - EC 2.7.10.2 (src-Family Kinases)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - Benzoquinones
MH  - Biological Transport/drug effects
MH  - Chalcone/analogs & derivatives/pharmacology
MH  - Chalcones
MH  - Deoxyglucose/pharmacokinetics
MH  - Electric Stimulation
MH  - Focal Adhesion Kinase 2/antagonists & inhibitors/metabolism
MH  - Genistein/pharmacology
MH  - Glucose/*pharmacokinetics
MH  - Glucose Transporter Type 4/metabolism
MH  - Hypoxia/physiopathology
MH  - In Vitro Techniques
MH  - Insulin/pharmacology
MH  - Lactams, Macrocyclic
MH  - Male
MH  - Muscle Contraction/*physiology
MH  - Muscle, Skeletal/drug effects/metabolism/*physiology
MH  - Phosphorylation/drug effects
MH  - Protein-Tyrosine Kinases/antagonists & inhibitors/*metabolism
MH  - Pyrimidines/pharmacology
MH  - Quinones/pharmacology
MH  - Rats
MH  - Rats, Wistar
MH  - Rifabutin/analogs & derivatives
MH  - src-Family Kinases/antagonists & inhibitors/metabolism
EDAT- 2005/09/15 09:00
MHDA- 2006/02/28 09:00
CRDT- 2005/09/15 09:00
PHST- 2005/09/15 09:00 [pubmed]
PHST- 2006/02/28 09:00 [medline]
PHST- 2005/09/15 09:00 [entrez]
AID - 00280.2005 [pii]
AID - 10.1152/ajpendo.00280.2005 [doi]
PST - ppublish
SO  - Am J Physiol Endocrinol Metab. 2006 Jan;290(1):E123-E128. doi: 
      10.1152/ajpendo.00280.2005. Epub 2005 Sep 13.