PMID- 16161280
OWN - NASA
STAT- MEDLINE
DCOM- 20050921
LR  - 20100324
IS  - 1002-0837 (Print)
IS  - 1002-0837 (Linking)
VI  - 18
IP  - 3
DP  - 2005 Jun
TI  - [The influence of circadian gene mPeriod2 on Lewis cancer cell].
PG  - 222-3
AB  - OBJECTIVE: To investigate the role of circadian gene mPeriod2 (mPer2) on tumor 
      proliferation and apoptosis. METHOD: The eukaryotic mPer2 expression vector 
      (pcDNA 3.1-mPer2) based on pcDNA 3.1 was transfected into the cultured Lewis 
      tumor cell by liposome method in vivo. The expression of mPer2 in transfected 
      Lewis tumor cell was detected by immunohistochemistry and flowcytometry. The 
      stable transfected cell line was screened by G418 and its proliferation and 
      apoptosis was examined by flowcytometry. RESULT: Expression of PERIOD2 protein in 
      pcDNA 3.1-mPer2 transfected Lewis tumor cell was proved by immunohistochemistry 
      and flowcytometry. Flowcytometry result of mPer2 expression Lewis tumor cell 
      showed less proliferation and high rate of apoptosis. CONCLUSION: mPer2 
      expression can suppress the proliferation of tumor cell and increase the 
      apoptosis of it.
FAU - Wang, Yue-qi
AU  - Wang YQ
FAU - Hua, Hui
AU  - Hua H
FAU - Zhu, Bin
AU  - Zhu B
FAU - Yin, Hua-hu
AU  - Yin HH
FAU - Liu, Yan-you
AU  - Liu YY
FAU - Yang, Chun-lei
AU  - Yang CL
FAU - Wang, Zheng-rong
AU  - Wang ZR
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Space Med Med Eng (Beijing)
JT  - Hang tian yi xue yu yi xue gong cheng = Space medicine & medical engineering
JID - 9425305
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Per2 protein, mouse)
RN  - 0 (Period Circadian Proteins)
RN  - 0 (Transcription Factors)
MH  - Animals
MH  - Apoptosis
MH  - Carcinoma, Lewis Lung/genetics
MH  - Cell Cycle Proteins
MH  - Cell Division
MH  - Circadian Rhythm/*genetics
MH  - DNA, Complementary/genetics
MH  - Flow Cytometry
MH  - Gene Expression
MH  - Mice
MH  - Nuclear Proteins/genetics/*metabolism
MH  - Period Circadian Proteins
MH  - Transcription Factors/genetics/*metabolism
MH  - Transfection
MH  - Tumor Cells, Cultured/immunology
OID - NASA: 00031507
EDAT- 2005/09/16 09:00
MHDA- 2005/09/22 09:00
CRDT- 2005/09/16 09:00
PHST- 2005/09/16 09:00 [pubmed]
PHST- 2005/09/22 09:00 [medline]
PHST- 2005/09/16 09:00 [entrez]
PST - ppublish
SO  - Space Med Med Eng (Beijing). 2005 Jun;18(3):222-3.