PMID- 1616135
OWN - NLM
STAT- MEDLINE
DCOM- 19920728
LR  - 20131121
IS  - 0003-2999 (Print)
IS  - 0003-2999 (Linking)
VI  - 75
IP  - 1
DP  - 1992 Jul
TI  - Effect of chloride transport blockade on the MAC of halothane in the rat.
PG  - 103-6
AB  - There is a growing evidence that central nervous system chloride transport via 
      gamma-aminobutyric acid (GABAA) related Cl- conductance or Cl-/HCO3- exchange 
      affects anesthetic requirements. To delineate the effects of GABAA-related Cl- 
      conductance blockade versus Cl-/HCO3- exchange inhibition, we determined the 
      change in minimum alveolar anesthetic concentration (MAC) of halothane in rats 
      after intracisternal infusion of 4,4'-diisothiocyano-2,2'-disulfonic acid 
      stilbene (DIDS). DIDS inhibits Cl-/HCO3- exchange transport in concentrations 
      greater than 1 microM and in GABAA-related Cl- channels in concentrations greater 
      than 0.1 mM. After control MAC determination, rats were given intracisternal mock 
      cerebrospinal fluid (n = 6), 1.0 microM DIDS (n = 8), or 1 mM DIDS (n = 8) at a 
      rate of 2 microL/min for 30 min. Mock cerebrospinal fluid did not change the MAC 
      of halothane. The MAC of halothane increased significantly (P less than 0.001) 
      from 0.96% +/- 0.02% to 1.11% +/- 0.03% (mean value +/- SEM) with 1 microM DIDS 
      and from 0.94% +/- 0.02% to 1.16% +/- 0.04% with 1 mM DIDS. The increases in MAC 
      with 1 microM and 1 mM DIDS were not statistically different. This suggests that 
      Cl-/HCO3- exchange inhibition increases halothane requirements, whereas 
      GABAA-related Cl- channel blockade does not.
FAU - Rich, G F
AU  - Rich GF
AD  - Department of Anesthesiology, University of Virginia Health Sciences Center, 
      Charlottesville 22908.
FAU - Sullivan, M P
AU  - Sullivan MP
FAU - Adams, J M
AU  - Adams JM
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Anesth Analg
JT  - Anesthesia and analgesia
JID - 1310650
RN  - 0 (Bicarbonates)
RN  - 0 (Chlorides)
RN  - 27816-59-7 (4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid)
RN  - Q1O6DSW23R (4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid)
RN  - UQT9G45D1P (Halothane)
SB  - IM
MH  - 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
MH  - 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid/*analogs & 
      derivatives/pharmacology
MH  - Animals
MH  - Bicarbonates/metabolism
MH  - Chlorides/*metabolism
MH  - Dose-Response Relationship, Drug
MH  - Halothane/*pharmacokinetics
MH  - Pulmonary Alveoli/drug effects/*metabolism
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Reference Values
EDAT- 1992/07/01 00:00
MHDA- 1992/07/01 00:01
CRDT- 1992/07/01 00:00
PHST- 1992/07/01 00:00 [pubmed]
PHST- 1992/07/01 00:01 [medline]
PHST- 1992/07/01 00:00 [entrez]
PST - ppublish
SO  - Anesth Analg. 1992 Jul;75(1):103-6.