PMID- 16162269
OWN - NLM
STAT- MEDLINE
DCOM- 20051110
LR  - 20181113
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 116
IP  - 2
DP  - 2005 Oct
TI  - Differential effects of CpG-DNA in Toll-like receptor-2/-4/-9 tolerance and 
      cross-tolerance.
PG  - 203-12
AB  - Lipopolysaccharide (LPS) tolerance is a state of refractoriness towards a second 
      stimulation by LPS after a preceding stimulation. LPS is recognized by Toll-like 
      receptor-4 (TLR-4), which belongs to a group of pattern recognition receptors 
      mediating activation of innate immunity by microbial components. To date, it is 
      not known in detail to what extent other TLR-dependent stimuli also induce 
      tolerance and whether preceding and challenging stimuli are interchangeable. We 
      have examined tolerance induction in detail for lipoteichoic acid (LTA), LPS and 
      CpG-DNA, which are recognized by TLR-2, -4 and -9, respectively. In RAW264.7 
      macrophages, all three stimuli induced tolerance towards a subsequent challenge 
      with the same stimulus used for priming, as well as cross-tolerance towards 
      subsequent challenge with other stimuli signalling via different TLRs. However, 
      whereas LPS/LTA cross-tolerance was also functional in an in vivo model of 
      galactosamine (GalN)-primed liver damage, pretreatment with CpG only protected 
      against GalN/CpG challenge and failed to induce cross-tolerance for LPS and LTA. 
      CpG-DNA pretreatment even enhanced tumour necrosis factor (TNF)-alpha production 
      and liver damage upon subsequent challenge with LPS or LTA. Stimulation with 
      CpG-DNA resulted in a peculiar sensitization for interferon (IFN)-gamma 
      secretion. The data indicate that, in contrast to in vitro macrophage 
      desensitization, the in vivo consequences of repeated TLR stimulation greatly 
      differ amongst different TLR ligands.
FAU - Dalpke, Alexander H
AU  - Dalpke AH
AD  - Institute of Medical Microbiology and Hygiene, Philipps-University Marburg, 
      Marburg, Germany. alexander.dalpke@med.uni-heidelberg.de
FAU - Lehner, Martin D
AU  - Lehner MD
FAU - Hartung, Thomas
AU  - Hartung T
FAU - Heeg, Klaus
AU  - Heeg K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (CPG-oligonucleotide)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Interleukin-18)
RN  - 0 (Ligands)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Teichoic Acids)
RN  - 0 (Tlr2 protein, mouse)
RN  - 0 (Tlr4 protein, mouse)
RN  - 0 (Tlr9 protein, mouse)
RN  - 0 (Toll-Like Receptor 2)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (Toll-Like Receptor 9)
RN  - 0 (Toll-Like Receptors)
RN  - 56411-57-5 (lipoteichoic acid)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Chemical and Drug Induced Liver Injury/immunology
MH  - CpG Islands
MH  - DNA-Binding Proteins/immunology
MH  - *Immune Tolerance
MH  - Interferon-gamma/biosynthesis
MH  - Interleukin-18/biosynthesis/immunology
MH  - Ligands
MH  - Lipopolysaccharides/immunology
MH  - Macrophages, Peritoneal/immunology
MH  - Membrane Glycoproteins/*immunology
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Oligodeoxyribonucleotides/*immunology
MH  - Receptors, Cell Surface/*immunology
MH  - Receptors, Immunologic/immunology
MH  - Teichoic Acids/immunology
MH  - Toll-Like Receptor 2
MH  - Toll-Like Receptor 4
MH  - Toll-Like Receptor 9
MH  - Toll-Like Receptors
PMC - PMC1817820
EDAT- 2005/09/16 09:00
MHDA- 2005/11/11 09:00
PMCR- 2006/10/01
CRDT- 2005/09/16 09:00
PHST- 2005/09/16 09:00 [pubmed]
PHST- 2005/11/11 09:00 [medline]
PHST- 2005/09/16 09:00 [entrez]
PHST- 2006/10/01 00:00 [pmc-release]
AID - IMM2211 [pii]
AID - 10.1111/j.1365-2567.2005.02211.x [doi]
PST - ppublish
SO  - Immunology. 2005 Oct;116(2):203-12. doi: 10.1111/j.1365-2567.2005.02211.x.