PMID- 16163545
OWN - NLM
STAT- MEDLINE
DCOM- 20060216
LR  - 20181113
IS  - 0009-5915 (Print)
IS  - 0009-5915 (Linking)
VI  - 114
IP  - 5
DP  - 2005 Nov
TI  - Chromosome reshuffling in birds of prey: the karyotype of the world's largest 
      eagle (Harpy eagle, Harpia harpyja) compared to that of the chicken (Gallus 
      gallus).
PG  - 338-43
AB  - Like various other diurnal birds of prey, the world's largest eagle, the Harpy 
      (Harpia harpyja), presents an atypical bird karyotype with 2n=58 chromosomes. 
      There is little knowledge about the dramatic changes in the genomic 
      reorganization of these species compared to other birds. Since recently, the 
      chicken provides a "default map" for various birds including the first genomic 
      DNA sequence of a bird species. Obviously, the gross division of the chicken 
      genome into relatively gene-poor macrochromosomes and predominantly gene-rich 
      microchromosomes has been conserved for more than 150 million years in most bird 
      species. Here, we present classical features of the Harpy eagle karyotype but 
      also chromosomal homologies between H. harpyja and the chicken by chromosome 
      painting and comparison to the chicken genome map. We used two different sets of 
      painting probes: (1) chicken chromosomes were divided into three size categories: 
      (a) macrochromosomes 1-5 and Z, (b) medium-sized chromosomes 6-10, and (c) 19 
      microchromosomes; (2) combinatorially labeled chicken chromosome paints 1-6 and 
      Z. Both probe sets were visualized on H. harpyja chromosomes by multicolor 
      fluorescence in situ hybridization (FISH). Our data show how the organization 
      into micro- and macrochromosomes has been lost in the Harpy eagle, seemingly 
      without any preference or constraints.
FAU - de Oliveira, Edivaldo H C
AU  - de Oliveira EH
AD  - Laboratorio de Citogenetica, Depto de Genetica, Universidade Federal do Para, 
      Belem, PA, Brazil.
FAU - Habermann, Felix A
AU  - Habermann FA
FAU - Lacerda, Oneida
AU  - Lacerda O
FAU - Sbalqueiro, Ives J
AU  - Sbalqueiro IJ
FAU - Wienberg, Johannes
AU  - Wienberg J
FAU - Muller, Stefan
AU  - Muller S
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20051112
PL  - Austria
TA  - Chromosoma
JT  - Chromosoma
JID - 2985138R
RN  - 0 (DNA Probes)
SB  - IM
MH  - Animals
MH  - Chickens/*genetics
MH  - Chromosome Painting/methods
MH  - Cytogenetic Analysis
MH  - DNA Probes
MH  - Eagles/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Repetitive Sequences, Nucleic Acid
MH  - Telomere/genetics
EDAT- 2005/09/16 09:00
MHDA- 2006/02/17 09:00
CRDT- 2005/09/16 09:00
PHST- 2004/12/14 00:00 [received]
PHST- 2005/05/19 00:00 [accepted]
PHST- 2005/05/18 00:00 [revised]
PHST- 2005/09/16 09:00 [pubmed]
PHST- 2006/02/17 09:00 [medline]
PHST- 2005/09/16 09:00 [entrez]
AID - 10.1007/s00412-005-0009-5 [doi]
PST - ppublish
SO  - Chromosoma. 2005 Nov;114(5):338-43. doi: 10.1007/s00412-005-0009-5. Epub 2005 Nov 
      12.