PMID- 16163698
OWN - NLM
STAT- MEDLINE
DCOM- 20051212
LR  - 20070724
IS  - 1552-4922 (Print)
IS  - 1552-4922 (Linking)
VI  - 67
IP  - 2
DP  - 2005 Oct
TI  - Chromosomal imbalances in laryngeal and hypopharyngeal cancers detected by 
      comparative genomic hybridization.
PG  - 151-60
AB  - BACKGROUND: The prognostic divergence of laryngeal and hypopharyngeal carcinomas 
      is well known. Hypopharyngeal tumors are characterized by frequent metastasis 
      formation and local recurrence, which is the source of the unfavorable prognosis 
      of this subtype. The aim of this study was to define chromosomal alterations 
      associated with the aggressive behavior of hypopharyngeal tumors. METHODS: 
      Twenty-nine head and neck squamous cell carcinomas (larynx n = 14 and hypopharynx 
      n = 15) were analyzed by comparative genomic hybridization (CGH). Fluorescence in 
      situ hybridization (FISH) was used to validate the CGH data and to compare the 
      amplification pattern of the most frequently altered gene (cyclin-D1, CCND1) 
      located on 11q13. RESULTS: The average number of genetic alterations was 
      significantly higher in the hypopharyngeal tumors (P = 0.02). A good correlation 
      of FISH and CGH data were seen. Gains on 11q13 were present in both subtypes, 
      whereas amplification of CCND1 was associated with the aggressive phenotype by 
      FISH. Chromosomal alteration, which was rarely detected in hypopharyngeal tumors 
      but was observed in more than 50% of laryngeal carcinomas, was 8q gain. 
      CONCLUSION: Our CGH and FISH data show that head and neck squamous cell 
      carcinomas contain complex cytogenetic alterations and further support the 
      hypothesis that different molecular pathways are responsible for the progression 
      of differently localized tumors of the upper aerodigestive tract.
FAU - Juhasz, Attila
AU  - Juhasz A
AD  - Medical and Health Science Center Department of Otorhinolaryngology and Head and 
      Neck Surgery, University of Debrecen, Debrecen, Hungary.
FAU - Balazs, Margit
AU  - Balazs M
FAU - Sziklay, Istvan
AU  - Sziklay I
FAU - Rakosy, Zsuzsa
AU  - Rakosy Z
FAU - Treszl, Andrea
AU  - Treszl A
FAU - Repassy, Gabor
AU  - Repassy G
FAU - Adany, Roza
AU  - Adany R
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cytometry A
JT  - Cytometry. Part A : the journal of the International Society for Analytical 
      Cytology
JID - 101235694
RN  - 136601-57-5 (Cyclin D1)
SB  - IM
MH  - Aged
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human, Pair 11/genetics
MH  - Cyclin D1/genetics
MH  - Female
MH  - Gene Dosage
MH  - Humans
MH  - Hypopharyngeal Neoplasms/*diagnosis/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Laryngeal Neoplasms/*diagnosis/*genetics
MH  - Male
MH  - Middle Aged
MH  - Nucleic Acid Hybridization
EDAT- 2005/09/16 09:00
MHDA- 2005/12/15 09:00
CRDT- 2005/09/16 09:00
PHST- 2005/09/16 09:00 [pubmed]
PHST- 2005/12/15 09:00 [medline]
PHST- 2005/09/16 09:00 [entrez]
AID - 10.1002/cyto.a.20165 [doi]
PST - ppublish
SO  - Cytometry A. 2005 Oct;67(2):151-60. doi: 10.1002/cyto.a.20165.