PMID- 16164636
OWN - NLM
STAT- MEDLINE
DCOM- 20051230
LR  - 20061115
IS  - 0085-2538 (Print)
IS  - 0085-2538 (Linking)
VI  - 68
IP  - 4
DP  - 2005 Oct
TI  - Matrix metalloproteinases and mesangial remodeling in light chain-related 
      glomerular damage.
PG  - 1590-603
AB  - BACKGROUND: Matrix metalloproteinases (MMPs) belong to the zinc endopeptidase 
      subgroup of the metalloproteinase superfamily and are primarily involved in 
      extracellular matrix (ECM) remodeling. Alterations of the mesangial ECM in 
      AL-amyloidosis (AL-Am) and light chain deposition disease (LCDD) are crucial in 
      their pathogeneses as two divergent entities. METHODS: Protein expression 
      patterns of five MMPs (MMP-1, 2, 3, 7, and 9) in renal tissues obtained from 
      autopsies and kidney biopsies, and cultured human mesangial cells (HMCs) treated 
      with light chains obtained from the urines of patients with AL-Am and LCDD were 
      analyzed. MMP mRNA expressions were determined in glomeruli following laser 
      capture microdissection and selective MMP microarray. Zymography was used to 
      assess MMP activity. RESULTS: The average glomerular MMP expression was 6 times 
      greater in AL-Am than LCDD and negative control renal tissues with different 
      expression profiles: MMP-1, 7 > 9 > 3 > 2, MMP-1 > 2, 9 > 3 > 7, and MMP-2, 3, 7 
      > 9 > 1, respectively. Microdissected glomeruli and HMCs treated with light 
      chains expressed higher levels of MMP mRNA and proteins in AL-Am than LCDD. 
      Zymography was used to assess activity demonstrating increased MMP-2 in AL-Am. 
      CONCLUSION: Altered expressions of MMPs play a key role in the pathogenesis of 
      AL-Am and LCDD. MMPs were more highly expressed in AL-Am compared to LCDD.
FAU - Keeling, John
AU  - Keeling J
AD  - Department of Pathology, Louisiana State University Health Sciences Center, 
      Shreveport, Louisiana 71130, USA.
FAU - Herrera, Guillermo A
AU  - Herrera GA
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Kidney Int
JT  - Kidney international
JID - 0323470
RN  - 0 (Collagen Type IV)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Immunoglobulin Light Chains)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tenascin)
RN  - 0 (Tissue Inhibitor of Metalloproteinases)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
RN  - EC 3.4.24.23 (Matrix Metalloproteinase 7)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
RN  - EC 3.4.24.7 (Matrix Metalloproteinase 1)
SB  - IM
MH  - Amyloidosis/metabolism/*pathology/*physiopathology
MH  - Blotting, Western
MH  - Cells, Cultured
MH  - Collagen Type IV/metabolism
MH  - Culture Media, Conditioned
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Glomerular Mesangium/*pathology/*physiology
MH  - Humans
MH  - Immunoglobulin Light Chains/metabolism
MH  - Immunohistochemistry
MH  - Matrix Metalloproteinase 1/genetics/metabolism
MH  - Matrix Metalloproteinase 2/genetics/metabolism
MH  - Matrix Metalloproteinase 3/genetics/metabolism
MH  - Matrix Metalloproteinase 7/genetics/metabolism
MH  - Matrix Metalloproteinase 9/genetics/metabolism
MH  - Matrix Metalloproteinases/*genetics/*metabolism
MH  - RNA, Messenger/analysis
MH  - Tenascin/metabolism
MH  - Tissue Inhibitor of Metalloproteinases/genetics
EDAT- 2005/09/17 09:00
MHDA- 2005/12/31 09:00
CRDT- 2005/09/17 09:00
PHST- 2005/09/17 09:00 [pubmed]
PHST- 2005/12/31 09:00 [medline]
PHST- 2005/09/17 09:00 [entrez]
AID - S0085-2538(15)51012-0 [pii]
AID - 10.1111/j.1523-1755.2005.00571.x [doi]
PST - ppublish
SO  - Kidney Int. 2005 Oct;68(4):1590-603. doi: 10.1111/j.1523-1755.2005.00571.x.