PMID- 16166251
OWN - NLM
STAT- MEDLINE
DCOM- 20060608
LR  - 20061115
IS  - 1066-5099 (Print)
IS  - 1066-5099 (Linking)
VI  - 24
IP  - 3
DP  - 2006 Mar
TI  - Gene-expression profiling of CD34+ hematopoietic cells expanded in a collagen I 
      matrix.
PG  - 494-500
AB  - CD34+ hematopoietic stem/progenitor cells (HSCs) reside in the bone marrow in 
      close proximity to the endosteal bone surface, surrounded by osteoblasts, stromal 
      cells, and various extracellular matrix molecules. We used a bioartificial matrix 
      of fibrillar collagen I, the major matrix component of bone, as a scaffold for ex 
      vivo expansion of HSCs. CD34+ HSCs were isolated from umbilical cord blood and 
      cultivated within reconstituted collagen I fibrils in the presence of fms-like 
      tyrosine kinase-3 ligand, stem cell factor, and interleukin (IL)-3. After 7 days 
      of culture, the cell number, number of colony-forming units (CFU-C), and 
      gene-expression profile of the cultured cells were assessed. Although the total 
      expansion factor of CD34+ cells was slightly lower when cells were cultivated in 
      the collagen I gel, the frequency of CFU-C was greater than in control suspension 
      cultures. Gene-expression analysis with microarray chip technology revealed the 
      upregulation of more than 50 genes in the presence of collagen I. Among these, 
      genes for several growth factors, cytokines, and chemokines (e.g., IL-8 and 
      macrophage inhibitory protein 1alpha) could be confirmed using quantitative 
      polymerase chain reaction. Furthermore, greater expression levels of the negative 
      cell-cycle regulator BTG2/TIS21 and an inhibitor of the mitogen-activated protein 
      kinase pathway, DUSP2, underline the regulatory role of the extracellular matrix. 
      Together, these data show that the expansion of CD34+ cord blood cells in a 
      culture system containing a three-dimensional collagen I matrix induces a 
      qualitative change in the gene-expression profile of cultivated HSCs.
FAU - Oswald, Joachim
AU  - Oswald J
AD  - Med. Klinik und Poliklinik I, University Hospital Carl Gustav Carus, Liebniz 
      Institute of Polymer Research Dresden, Germany.
FAU - Steudel, Christine
AU  - Steudel C
FAU - Salchert, Katrin
AU  - Salchert K
FAU - Joergensen, Brigitte
AU  - Joergensen B
FAU - Thiede, Christian
AU  - Thiede C
FAU - Ehninger, Gerhard
AU  - Ehninger G
FAU - Werner, Carsten
AU  - Werner C
FAU - Bornhauser, Martin
AU  - Bornhauser M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050915
PL  - England
TA  - Stem Cells
JT  - Stem cells (Dayton, Ohio)
JID - 9304532
RN  - 0 (Antigens, CD34)
RN  - 0 (Collagen Type I)
RN  - 0 (Cytokines)
SB  - IM
MH  - *Antigens, CD34
MH  - Cell Culture Techniques
MH  - *Cell Differentiation
MH  - Cells, Cultured
MH  - *Collagen Type I
MH  - Cytokines/pharmacology
MH  - Fetal Blood/cytology/*physiology
MH  - Gene Expression Profiling/methods
MH  - Gene Expression Regulation/drug effects/*physiology
MH  - Hematopoietic Stem Cells/*physiology/ultrastructure
MH  - Humans
MH  - Microscopy, Electron, Scanning/methods
MH  - Oligonucleotide Array Sequence Analysis/methods
EDAT- 2005/09/17 09:00
MHDA- 2006/06/09 09:00
CRDT- 2005/09/17 09:00
PHST- 2005/09/17 09:00 [pubmed]
PHST- 2006/06/09 09:00 [medline]
PHST- 2005/09/17 09:00 [entrez]
AID - 2005-0276 [pii]
AID - 10.1634/stemcells.2005-0276 [doi]
PST - ppublish
SO  - Stem Cells. 2006 Mar;24(3):494-500. doi: 10.1634/stemcells.2005-0276. Epub 2005 
      Sep 15.