PMID- 16168606
OWN - NLM
STAT- MEDLINE
DCOM- 20060427
LR  - 20220408
IS  - 0731-7085 (Print)
IS  - 0731-7085 (Linking)
VI  - 40
IP  - 3
DP  - 2006 Feb 24
TI  - Identification and determination of nucleosides in Cordyceps sinensis and its 
      substitutes by high performance liquid chromatography with mass spectrometric 
      detection.
PG  - 623-30
AB  - Cordyceps sinensis (Cs) is a well-known traditional Chinese medicine (TCM) and 
      Cordyceps mycelia (Cm), a cultured Cordyceps, is a substitute for Cordyceps 
      sinensis. The most important active components in them are nucleosides. A high 
      selective, sensitive and accurate high performance liquid chromatography method 
      with photodiode array detection (DAD) and mass spectrometric detection has been 
      developed for simultaneous separation, identification and quantification of 
      nucleosides in Cs and Cm using a mobile phase including (A) ammonium acetate (40 
      mM, pH 5.2) and (B) methanol with a gradient system on a 2.0 mm x 150 mm Shimadzu 
      VP-ODS column. The presence of each nucleoside in Cs and Cm was ascertained by 
      comparison of MS data, UV spectra and retention time with standards. LC/ESI-MS in 
      selective ion monitoring (SIM) mode were used for the quantification of 
      nucleosides in Cs and Cm. 2-Chloroadenosine was used as internal standard for 
      this assay. The precisions and accuracies were in the range of 1.5-5.3% and -3.5 
      to 5.0%, respectively. The limits of detection and quantification for nucleosides 
      were in the order of 0.1-0.6 microg ml(-1) and 0.5-2.0 microg ml(-1), 
      respectively. The recoveries were in the range of 92.0-107.0%. With the developed 
      method, the concentrations of nucleosides in Cs and Cm from different sources 
      were determined. Cs, characterized with far lower concentration of adenosine and 
      cordycepin than Cm, can be very easy to distinguish from Cm. This reliable method 
      would be useful for the study and quality control of Cordyceps sinensis and its 
      substitutes.
FAU - Guo, Fang-Qiu
AU  - Guo FQ
AD  - Research Center of Modernization of Chinese Herbal Medicines, College of 
      Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR 
      China.
FAU - Li, Ai
AU  - Li A
FAU - Huang, Lan-Fang
AU  - Huang LF
FAU - Liang, Yi-Zeng
AU  - Liang YZ
FAU - Chen, Ben-Mei
AU  - Chen BM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050915
PL  - England
TA  - J Pharm Biomed Anal
JT  - Journal of pharmaceutical and biomedical analysis
JID - 8309336
RN  - 0 (Nucleosides)
SB  - IM
MH  - Chromatography, High Pressure Liquid
MH  - Chromatography, Liquid
MH  - Cordyceps/*chemistry
MH  - Mass Spectrometry
MH  - Nucleosides/*analysis
MH  - Reference Standards
MH  - Reproducibility of Results
MH  - Spectrometry, Mass, Electrospray Ionization
EDAT- 2005/09/20 09:00
MHDA- 2006/04/28 09:00
CRDT- 2005/09/20 09:00
PHST- 2005/05/20 00:00 [received]
PHST- 2005/07/28 00:00 [revised]
PHST- 2005/07/29 00:00 [accepted]
PHST- 2005/09/20 09:00 [pubmed]
PHST- 2006/04/28 09:00 [medline]
PHST- 2005/09/20 09:00 [entrez]
AID - S0731-7085(05)00535-2 [pii]
AID - 10.1016/j.jpba.2005.07.034 [doi]
PST - ppublish
SO  - J Pharm Biomed Anal. 2006 Feb 24;40(3):623-30. doi: 10.1016/j.jpba.2005.07.034. 
      Epub 2005 Sep 15.