PMID- 16169494
OWN - NLM
STAT- MEDLINE
DCOM- 20060418
LR  - 20160610
IS  - 0960-9822 (Print)
IS  - 0960-9822 (Linking)
VI  - 15
IP  - 18
DP  - 2005 Sep 20
TI  - Intraflagellar transport is required for the vectorial movement of TRPV channels 
      in the ciliary membrane.
PG  - 1695-9
AB  - The membranes of all eukaryotic motile (9 + 2) and immotile primary (9 + 0) cilia 
      harbor channels and receptors involved in sensory transduction (reviewed by). 
      These membrane proteins are transported from the cytoplasm onto the ciliary 
      membrane by vesicles targeted for exocytosis at a point adjacent to the ciliary 
      basal body. Here, we use time-lapse fluorescence microscopy to demonstrate that 
      select GFP-tagged sensory receptors undergo rapid vectorial transport along the 
      entire length of the cilia of Caenorhabditis elegans sensory neurons. Transient 
      receptor potential vanilloid (TRPV) channels OSM-9 and OCR-2 move in ciliary 
      membranes at rates comparable to the intraflagellar transport (IFT) machinery 
      located between the membrane and the underlying axonemal microtubules. OSM-9 
      motility is disrupted in certain IFT mutant backgrounds. Surprisingly, motility 
      of transient receptor potential polycystin (TRPP) channel PKD-2 (polycystic 
      kidney disease-2), a mechano-receptor, was not detected. Our study demonstrates 
      that IFT, previously shown to be necessary for transport of axonemal components, 
      is also involved in the motility of TRPV membrane protein movement along cilia of 
      C. elegans sensory cells.
FAU - Qin, Hongmin
AU  - Qin H
AD  - Department of Molecular, Cellular and Developmental Biology, Yale University, New 
      Haven, Connecticut 06511, USA.
FAU - Burnette, Dylan T
AU  - Burnette DT
FAU - Bae, Young-Kyung
AU  - Bae YK
FAU - Forscher, Paul
AU  - Forscher P
FAU - Barr, Maureen M
AU  - Barr MM
FAU - Rosenbaum, Joel L
AU  - Rosenbaum JL
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Curr Biol
JT  - Current biology : CB
JID - 9107782
RN  - 0 (Caenorhabditis elegans Proteins)
RN  - 0 (Ion Channels)
RN  - 0 (Membrane Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (OCR-2 protein, C elegans)
RN  - 0 (OSM-9 protein, C elegans)
RN  - 0 (TRPP Cation Channels)
RN  - 0 (TRPV Cation Channels)
RN  - 0 (Transient Receptor Potential Channels)
RN  - 0 (polycystic kidney disease 2 protein)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Animals
MH  - Biological Transport/physiology
MH  - Caenorhabditis elegans/*physiology
MH  - Caenorhabditis elegans Proteins/*metabolism
MH  - Cell Membrane/*metabolism
MH  - Cilia/*metabolism
MH  - Green Fluorescent Proteins
MH  - Ion Channels/*metabolism
MH  - Membrane Proteins/metabolism
MH  - Microscopy, Fluorescence
MH  - Nerve Tissue Proteins/*metabolism
MH  - Neurons, Afferent/*metabolism/physiology
MH  - TRPP Cation Channels
MH  - TRPV Cation Channels
MH  - Transient Receptor Potential Channels/*metabolism
EDAT- 2005/09/20 09:00
MHDA- 2006/04/19 09:00
CRDT- 2005/09/20 09:00
PHST- 2005/06/15 00:00 [received]
PHST- 2005/08/01 00:00 [revised]
PHST- 2005/08/09 00:00 [accepted]
PHST- 2005/09/20 09:00 [pubmed]
PHST- 2006/04/19 09:00 [medline]
PHST- 2005/09/20 09:00 [entrez]
AID - S0960-9822(05)00966-8 [pii]
AID - 10.1016/j.cub.2005.08.047 [doi]
PST - ppublish
SO  - Curr Biol. 2005 Sep 20;15(18):1695-9. doi: 10.1016/j.cub.2005.08.047.