PMID- 16169505
OWN - NLM
STAT- MEDLINE
DCOM- 20051114
LR  - 20181201
IS  - 1074-7613 (Print)
IS  - 1074-7613 (Linking)
VI  - 23
IP  - 3
DP  - 2005 Sep
TI  - Cutaneous CXCL14 targets blood precursors to epidermal niches for Langerhans cell 
      differentiation.
PG  - 331-42
AB  - Dendritic cells (DCs) are major constituents of peripheral tissues, where they 
      control immunity to foreign and self-antigens. The process of continuous DC 
      renewal under homeostatic conditions is largely undefined. Here, we demonstrate 
      that CD14+ DC precursors, either derived from CD34+ hematopoietic progenitor 
      cells or isolated from blood, were attracted by the chemokine CXCL14, which is 
      constitutively produced in healthy skin and other epithelial tissues. In a tissue 
      model we show that human epidermal equivalents profoundly affected CD14+ DC 
      precursors, including their suprabasal positioning and survival as well as their 
      differentiation into Langerhans cell-like cells with potent antigen-presentation 
      functions. Our model assigns unprecedented roles to CXCL14 and epidermal tissue 
      as attractant and niche of differentiation, respectively, in the renewal of 
      Langerhans cells under steady-state conditions.
FAU - Schaerli, Patrick
AU  - Schaerli P
AD  - Institute of Cell Biology, University of Bern, CH-3012 Bern, Switzerland.
FAU - Willimann, Katharina
AU  - Willimann K
FAU - Ebert, Lisa M
AU  - Ebert LM
FAU - Walz, Alfred
AU  - Walz A
FAU - Moser, Bernhard
AU  - Moser B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Immunity
JT  - Immunity
JID - 9432918
RN  - 0 (CXCL14 protein, human)
RN  - 0 (Chemokines, CXC)
SB  - IM
MH  - Cell Differentiation/*immunology
MH  - Cell Movement/*immunology
MH  - Cells, Cultured
MH  - Chemokines, CXC/*immunology/metabolism
MH  - Coculture Techniques
MH  - Epidermal Cells
MH  - Epidermis/*immunology
MH  - Flow Cytometry
MH  - Hematopoietic Stem Cells/*immunology/metabolism
MH  - Humans
MH  - Immunohistochemistry
MH  - Langerhans Cells/*cytology/immunology/metabolism
MH  - Microscopy, Confocal
MH  - Models, Immunological
EDAT- 2005/09/20 09:00
MHDA- 2005/11/15 09:00
CRDT- 2005/09/20 09:00
PHST- 2004/12/06 00:00 [received]
PHST- 2005/08/24 00:00 [revised]
PHST- 2005/08/25 00:00 [accepted]
PHST- 2005/09/20 09:00 [pubmed]
PHST- 2005/11/15 09:00 [medline]
PHST- 2005/09/20 09:00 [entrez]
AID - S1074-7613(05)00273-6 [pii]
AID - 10.1016/j.immuni.2005.08.012 [doi]
PST - ppublish
SO  - Immunity. 2005 Sep;23(3):331-42. doi: 10.1016/j.immuni.2005.08.012.