PMID- 16170542 OWN - NLM STAT- MEDLINE DCOM- 20060601 LR - 20201209 IS - 0017-8470 (Print) IS - 0017-8470 (Linking) VI - 57 IP - 2 DP - 2006 Feb TI - [Species differentiation of yeasts of the genus Malassezia with Fourier transform infrared spectroscopy]. PG - 127-36 AB - BACKGROUND: 83 Malassezia strains (65 wild isolates and 18 reference strains) were differentiated to the species level using conventional methods including morphological and biochemical features. These strains were further analyzed by Fourier transform infrared spectroscopy (FT-IRS). RESULTS: FT-IRS analysis allowed a clear separation of Malassezia strains according to species-specific cluster formation. The main differences were found between Malassezia furfur and other Malassezia species. In addition, within the species Malassezia furfur, a separation in two similar groups could be demonstrated. A disadvantage of FT-IRS is the relatively expensive apparatus. A great advantage is the speed and simplicity of the procedure, producing results within minutes. CONCLUSION: In pityriasis versicolor, Malassezia globosa was the dominant species found in 62% of cases. In addition, Malassezia furfur was found in 60% of dandruff cases. FAU - Kalinowska-Pujdak, A AU - Kalinowska-Pujdak A AD - Klinik fur Dermatologie, Venerologie und Allergologie, Universitatsklinikum Leipzig. FAU - Schmalreck, A AU - Schmalreck A FAU - Haustein, U-F AU - Haustein UF FAU - Nenoff, P AU - Nenoff P LA - ger PT - Clinical Trial PT - Journal Article TT - Speziesdifferenzierung von Hefen der Gattung Malassezia mittels Fourier-Transform-Infrarot-Spektroskopie. PL - Germany TA - Hautarzt JT - Der Hautarzt; Zeitschrift fur Dermatologie, Venerologie, und verwandte Gebiete JID - 0372755 SB - IM MH - Dermatomycoses/*diagnosis/*microbiology MH - Humans MH - Malassezia/*classification/*isolation & purification MH - Species Specificity MH - Spectroscopy, Fourier Transform Infrared/*methods EDAT- 2005/09/20 09:00 MHDA- 2006/06/02 09:00 CRDT- 2005/09/20 09:00 PHST- 2005/09/20 09:00 [pubmed] PHST- 2006/06/02 09:00 [medline] PHST- 2005/09/20 09:00 [entrez] AID - 10.1007/s00105-005-1018-2 [doi] PST - ppublish SO - Hautarzt. 2006 Feb;57(2):127-36. doi: 10.1007/s00105-005-1018-2.