PMID- 16171163
OWN - NLM
STAT- MEDLINE
DCOM- 20051129
LR  - 20220408
IS  - 1050-6586 (Print)
IS  - 1050-6586 (Linking)
VI  - 19
IP  - 4
DP  - 2005 Jul-Aug
TI  - Staphylococcal exotoxins and nasal polyposis: analysis of systemic and local 
      responses.
PG  - 327-33
AB  - BACKGROUND: Staphylococcal exotoxins have been implicated in the pathogenesis of 
      several chronic inflammatory diseases including atopic dermatitis (AD), asthma, 
      and, most recently, chronic rhinosinusitis with nasal polyposis (CRS/NP). In 
      severe AD, these toxins act both as superantigens (SAg), triggering massive 
      T-cell activation, and as conventional allergens, triggering toxin-specific 
      immunoglobulin E (IgE) in the serum. In CRS/NP, evidence for both processes has 
      been reported but it is unclear whether these processes are linked. The aim of 
      this study was to correlate SAg activity as inferred by staphylococcal-specific 
      T-cell receptor (TCR) V-beta expansion in the polyp and blood of CRS/NP patients 
      with staphylococcal-specific anti-IgE antibodies in the serum. METHODS: IgE 
      antibodies to staphylococcal exotoxin A (SEA), staphylococcal exotoxin B (SEB), 
      and toxic shock syndrome toxin (TSST) 1 were measured in the serum of 12 
      individuals with CRS/NP before functional endoscopic sinus surgery. Flow 
      cytometry was used to analyze the SEA, SEB, and TSST-1-specific TCR V-beta 
      domains on the T cells from the polyp and blood of these patients. RESULTS: Serum 
      SEA-, SEB-, and TSST-1-specific IgE antibodies were detected in 0/12 (0%), 6/12 
      (50.0%), and 9/12 (75%) of CRS/NP patients, respectively. Evidence of SAg effect 
      in the polyp lymphocytes (TCR V-beta expansion in both CD4+ and CD8+ subsets) was 
      noted in 7/12 (58.3%) patients. Five of 6 CRS/NP patients had overlapping 
      evidence of a systemic IgE response and TCR V-beta expansion, suggestive of 
      exposure to the same exotoxin. No patients had evidence a SAg effect in blood 
      lymphocytes. Nine of 12 subjects also had coexistent asthma. CONCLUSION: These 
      results provide evidence for a local SAg effect in 7/12 (58.3%) polyp patients 
      and establish a positive correlation of V-beta expansion with the presence of 
      corresponding toxin-specific IgE in the serum.
FAU - Tripathi, Anju
AU  - Tripathi A
AD  - Division of Allergy-Immunology, Northwestern University Feinberg School of 
      Medicine, Chicago, Illinois, USA.
FAU - Kern, Robert
AU  - Kern R
FAU - Conley, David B
AU  - Conley DB
FAU - Seiberling, Kristin
AU  - Seiberling K
FAU - Klemens, Julie C
AU  - Klemens JC
FAU - Harris, Kathleen E
AU  - Harris KE
FAU - Suh, Lydia
AU  - Suh L
FAU - Huang, Jie
AU  - Huang J
FAU - Grammer, Leslie C
AU  - Grammer LC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Rhinol
JT  - American journal of rhinology
JID - 8807268
RN  - 0 (Exotoxins)
RN  - 0 (Receptors, Antigen, T-Cell, alpha-beta)
RN  - 0 (Superantigens)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Exotoxins
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - Immunoglobulin E/blood
MH  - Male
MH  - Middle Aged
MH  - Nasal Polyps/immunology/*microbiology/*physiopathology
MH  - Phenotype
MH  - Receptors, Antigen, T-Cell, alpha-beta/immunology
MH  - Rhinitis/microbiology
MH  - Sinusitis/microbiology
MH  - Staphylococcus/*immunology/*pathogenicity
MH  - *Superantigens
EDAT- 2005/09/21 09:00
MHDA- 2005/12/13 09:00
CRDT- 2005/09/21 09:00
PHST- 2005/09/21 09:00 [pubmed]
PHST- 2005/12/13 09:00 [medline]
PHST- 2005/09/21 09:00 [entrez]
PST - ppublish
SO  - Am J Rhinol. 2005 Jul-Aug;19(4):327-33.