PMID- 16171919
OWN - NLM
STAT- MEDLINE
DCOM- 20060314
LR  - 20171116
IS  - 0300-483X (Print)
IS  - 0300-483X (Linking)
VI  - 217
IP  - 1
DP  - 2006 Jan 5
TI  - Urinary and amniotic fluid levels of phthalate monoesters in rats after the oral 
      administration of di(2-ethylhexyl) phthalate and di-n-butyl phthalate.
PG  - 22-30
AB  - Two studies were designed to examine amniotic fluid and maternal urine 
      concentrations of the di(2-ethylhexyl) phthalate (DEHP) metabolite 
      mono(2-ethylhexyl) phthalate (MEHP) and the di-n-butyl phthalate (DBP) metabolite 
      monobutyl phthalate (MBP) after administration of DEHP and DBP during pregnancy. 
      In the first study, pregnant Sprague-Dawley rats were administered 0, 11, 33, 
      100, or 300 mg DEHP/kg/day by oral gavage starting on gestational day (GD) 7. In 
      the second study, DBP was administered by oral gavage to pregnant Sprague-Dawley 
      rats at doses of 0, 100, or 250 mg/kg/day starting on GD 13. Maternal urine and 
      amniotic fluid were collected and analyzed to determine the free and 
      glucuronidated levels of MEHP and MBP. In urine, MEHP and MBP were mostly 
      glucuronidated. By contrast, free MEHP and free MBP predominated in amniotic 
      fluid. Statistically significant correlations were found between maternal DEHP 
      dose and total maternal urinary MEHP (p=0.0117), and between maternal DEHP dose 
      and total amniotic fluid MEHP levels (p=0.0021). Total maternal urinary MEHP and 
      total amniotic fluid MEHP levels were correlated (Pearson correlation 
      coefficient=0.968). Statistically significant differences were found in amniotic 
      MBP levels between animals within the same DBP dose treatment group (p<0.0001) 
      and between animals in different dose treatment groups (p<0.0001). Amniotic fluid 
      MBP levels increased with increasing DBP doses, and high variability in maternal 
      urinary levels of MBP between rats was observed. Although no firm conclusions 
      could be drawn from the urinary MBP data, the MEHP results suggest that maternal 
      urinary MEHP levels may be useful surrogate markers for fetal exposure to DEHP.
FAU - Calafat, Antonia M
AU  - Calafat AM
AD  - Division of Laboratory Sciences, National Center for Environmental Health, 
      Centers for Disease Control and Prevention, 4770 Buford Hwy., NE, Mailstop F17, 
      Atlanta, GA 30341, USA. ACalafat@cdc.gov
FAU - Brock, John W
AU  - Brock JW
FAU - Silva, Manori J
AU  - Silva MJ
FAU - Gray, L Earl Jr
AU  - Gray LE Jr
FAU - Reidy, John A
AU  - Reidy JA
FAU - Barr, Dana B
AU  - Barr DB
FAU - Needham, Larry L
AU  - Needham LL
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20050919
PL  - Ireland
TA  - Toxicology
JT  - Toxicology
JID - 0361055
RN  - 0 (Glucuronides)
RN  - 0 (Phthalic Acids)
RN  - 2286E5R2KE (Dibutyl Phthalate)
RN  - AYI8EX34EU (Creatinine)
RN  - C42K0PH13C (Diethylhexyl Phthalate)
RN  - FU2EWB60RT (mono-(2-ethylhexyl)phthalate)
RN  - ZI46LWZ45G (monobutyl phthalate)
SB  - IM
MH  - Administration, Oral
MH  - Amniotic Fluid/*chemistry/drug effects/metabolism
MH  - Animals
MH  - Creatinine/urine
MH  - Dibutyl Phthalate/administration & dosage/metabolism/*pharmacokinetics
MH  - Diethylhexyl Phthalate/administration & dosage/*analogs & 
      derivatives/metabolism/*pharmacokinetics/urine
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - Gestational Age
MH  - Glucuronides/urine
MH  - Male
MH  - Phthalic Acids/*urine
MH  - Pregnancy
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2005/09/21 09:00
MHDA- 2006/03/15 09:00
CRDT- 2005/09/21 09:00
PHST- 2005/06/30 00:00 [received]
PHST- 2005/08/09 00:00 [revised]
PHST- 2005/08/15 00:00 [accepted]
PHST- 2005/09/21 09:00 [pubmed]
PHST- 2006/03/15 09:00 [medline]
PHST- 2005/09/21 09:00 [entrez]
AID - S0300-483X(05)00378-1 [pii]
AID - 10.1016/j.tox.2005.08.013 [doi]
PST - ppublish
SO  - Toxicology. 2006 Jan 5;217(1):22-30. doi: 10.1016/j.tox.2005.08.013. Epub 2005 
      Sep 19.