PMID- 16171942
OWN - NLM
STAT- MEDLINE
DCOM- 20060124
LR  - 20161124
IS  - 0303-7207 (Print)
IS  - 0303-7207 (Linking)
VI  - 242
IP  - 1-2
DP  - 2005 Oct 20
TI  - 5-(3,5-Di-tert-butyl-4-hydroxybenzylidene) thiazolidine-2,4-dione modulates 
      peroxisome proliferators-activated receptor gamma in 3T3-L1 adipocytes: roles as 
      a PPARgamma ligand.
PG  - 96-102
AB  - Based on the binding between peroxisome proliferators-activated receptor gamma 
      (PPARgamma) and steroid receptor co-activator-1 (SRC-1), an enzyme-linked 
      immunosorbent assay (ELISA) was used to screen new PPARgamma ligands from various 
      benzylidinethiazole derivatives, which have anti-inflammatory activity. Among 
      those derivatives, 5-(3,5-di-tert-butyl-4-hydroxybenzylidene) 
      thiazolidine-2,4-dione (BTZD) increased the binding between PPARgamma and SRC-1. 
      BTZD was found to induce adipogenesis and PPARgamma trans-activation in 3T3-L1 
      pre-adipocyte, and increased the binding between PPARgamma and SRC-1 in in vitro 
      binding assay and complex consisting of PPARgamma and SRC-1 in the 
      co-immunoprecipitaion. Chromatin immunoprecipitation (ChIP) analysis revealed 
      that BTZD induced the binding of PPARgamma-SRC-1 complex to PPAR response element 
      (PPRE) in the same pattern of other PPARgamma ligand. From these studies, we have 
      identified and studied the function of a new PPARgamma ligand, BTZD. We suggest 
      that BTZD can be used as a modulator of PPARgamma. This study applying ELISA and 
      ChIP assay can offer new methods to screen PPARgamma ligand and understand the 
      effects of PPARgamma ligands on inflammation.
FAU - Cho, Min-Chul
AU  - Cho MC
AD  - Laboratory of Cell Biology, Korea Research Institute of Bioscience and 
      Biotechnology, P.O. Box 115, Daejeon 305-600, Republic of Korea.
FAU - Lee, Woo Song
AU  - Lee WS
FAU - Hong, Jin-Tae
AU  - Hong JT
FAU - Park, Se-Won
AU  - Park SW
FAU - Moon, Dong-Cheul
AU  - Moon DC
FAU - Paik, Sang-Gi
AU  - Paik SG
FAU - Yoon, Do-Young
AU  - Yoon DY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Mol Cell Endocrinol
JT  - Molecular and cellular endocrinology
JID - 7500844
RN  - 0 (5-(3,5-di-tert-butyl-4-hydroxybenzylidene)thiazolidine-2,4-dione)
RN  - 0 (Ligands)
RN  - 0 (PPAR gamma)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Thiazolidinediones)
RN  - 0 (Transcription Factors)
RN  - EC 2.3.1.48 (Histone Acetyltransferases)
RN  - EC 2.3.1.48 (Ncoa1 protein, mouse)
RN  - EC 2.3.1.48 (Nuclear Receptor Coactivator 1)
SB  - IM
MH  - 3T3-L1 Cells
MH  - Adipocytes/*drug effects/metabolism
MH  - Animals
MH  - Binding Sites
MH  - Fibroblasts/cytology/drug effects
MH  - Genes, Reporter
MH  - Histone Acetyltransferases
MH  - Ligands
MH  - Lipid Metabolism/drug effects
MH  - Mice
MH  - Nuclear Receptor Coactivator 1
MH  - PPAR gamma/*metabolism
MH  - Promoter Regions, Genetic/genetics
MH  - Protein Binding/drug effects
MH  - Recombinant Fusion Proteins
MH  - Thiazolidinediones/*metabolism/*pharmacology
MH  - Transcription Factors/metabolism
MH  - Transcriptional Activation/drug effects/genetics
EDAT- 2005/09/21 09:00
MHDA- 2006/01/25 09:00
CRDT- 2005/09/21 09:00
PHST- 2005/07/19 00:00 [received]
PHST- 2005/08/04 00:00 [accepted]
PHST- 2005/09/21 09:00 [pubmed]
PHST- 2006/01/25 09:00 [medline]
PHST- 2005/09/21 09:00 [entrez]
AID - S0303-7207(05)00277-7 [pii]
AID - 10.1016/j.mce.2005.08.005 [doi]
PST - ppublish
SO  - Mol Cell Endocrinol. 2005 Oct 20;242(1-2):96-102. doi: 10.1016/j.mce.2005.08.005.