PMID- 16172256
OWN - NLM
STAT- MEDLINE
DCOM- 20051115
LR  - 20220129
IS  - 0022-1007 (Print)
IS  - 1540-9538 (Electronic)
IS  - 0022-1007 (Linking)
VI  - 202
IP  - 6
DP  - 2005 Sep 19
TI  - The structure of human CD23 and its interactions with IgE and CD21.
PG  - 751-60
AB  - The low-affinity immunoglobulin E (IgE) receptor, CD23 (FcepsilonRII), binds both 
      IgE and CD21 and, through these interactions, regulates the synthesis of IgE, the 
      antibody isotype that mediates the allergic response. We have determined the 
      three-dimensional structure of the C-type lectin domain of CD23 in solution by 
      nuclear magnetic resonance spectroscopy. An analysis of concentration-dependent 
      chemical shift perturbations have allowed us to identify the residues engaged in 
      self-association to the trimeric state, whereas ligand-induced changes have 
      defined the binding sites for IgE and CD21. The results further reveal that CD23 
      can bind both ligands simultaneously. Despite the C-type lectin domain structure, 
      none of the interactions require calcium. We also find that IgE and CD23 can 
      interact to form high molecular mass multimeric complexes. The interactions that 
      we have described provide a solution to the paradox that CD23 is involved in both 
      up- and down-regulation of IgE and provide a structural basis for the development 
      of inhibitors of allergic disease.
FAU - Hibbert, Richard G
AU  - Hibbert RG
AD  - Laboratory of Molecular Biophysics, Department of Biochemistry, University of 
      Oxford, Oxford OX1 3QU, England, UK.
FAU - Teriete, Peter
AU  - Teriete P
FAU - Grundy, Gabrielle J
AU  - Grundy GJ
FAU - Beavil, Rebecca L
AU  - Beavil RL
FAU - Reljic, Rajko
AU  - Reljic R
FAU - Holers, V Michael
AU  - Holers VM
FAU - Hannan, Jonathan P
AU  - Hannan JP
FAU - Sutton, Brian J
AU  - Sutton BJ
FAU - Gould, Hannah J
AU  - Gould HJ
FAU - McDonnell, James M
AU  - McDonnell JM
LA  - eng
GR  - WT_/Wellcome Trust/United Kingdom
GR  - G0200486/MRC_/Medical Research Council/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Exp Med
JT  - The Journal of experimental medicine
JID - 2985109R
RN  - 0 (Lectins)
RN  - 0 (Ligands)
RN  - 0 (Receptors, Complement 3d)
RN  - 0 (Receptors, IgE)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Binding Sites
MH  - Calcium/metabolism
MH  - Down-Regulation/immunology
MH  - Humans
MH  - Immunoglobulin E/biosynthesis/*metabolism
MH  - Lectins/physiology
MH  - Ligands
MH  - Magnetic Resonance Spectroscopy
MH  - Protein Binding
MH  - Protein Structure, Tertiary
MH  - Receptors, Complement 3d/*metabolism
MH  - Receptors, IgE/*chemistry/*metabolism
MH  - Surface Plasmon Resonance
MH  - Up-Regulation/immunology
PMC - PMC2212946
EDAT- 2005/09/21 09:00
MHDA- 2005/11/16 09:00
PMCR- 2006/03/19
CRDT- 2005/09/21 09:00
PHST- 2005/09/21 09:00 [pubmed]
PHST- 2005/11/16 09:00 [medline]
PHST- 2005/09/21 09:00 [entrez]
PHST- 2006/03/19 00:00 [pmc-release]
AID - jem.20050811 [pii]
AID - 20050811 [pii]
AID - 10.1084/jem.20050811 [doi]
PST - ppublish
SO  - J Exp Med. 2005 Sep 19;202(6):751-60. doi: 10.1084/jem.20050811.