PMID- 16177293
OWN - NLM
STAT- MEDLINE
DCOM- 20051128
LR  - 20191210
IS  - 0019-9567 (Print)
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Linking)
VI  - 73
IP  - 10
DP  - 2005 Oct
TI  - Transcriptional profiling of target of RNAIII-activating protein, a master 
      regulator of staphylococcal virulence.
PG  - 6220-8
AB  - Staphylococcus aureus is a gram-positive bacterium that is part of the normal 
      healthy flora but that can become virulent and cause infections by producing 
      biofilms and toxins. The production of virulence factors is regulated by 
      cell-cell communication (quorum sensing) through the histidine phosphorylation of 
      target of RNAIII-activating protein (TRAP), which is a 21-kDa protein that is 
      highly conserved among staphylococci. Using microarray analysis, we show here 
      that the expression and phosphorylation of TRAP upregulate the expression of 
      most, if not all, toxins known to date, as well as their global regulator agr. In 
      addition, we show here that the expression and phosphorylation of TRAP are also 
      necessary for the expression of genes known to be necessary for the survival of 
      the bacteria in a biofilm, like arc, pyr, and ure. TRAP is thus demonstrated to 
      be a master regulator of staphylococcal pathogenesis.
FAU - Korem, Moshe
AU  - Korem M
AD  - Department of Human Microbiology, Sackler School of Medicine, Tel Aviv 
      University, Israel.
FAU - Gov, Yael
AU  - Gov Y
FAU - Kiran, Madanahally D
AU  - Kiran MD
FAU - Balaban, Naomi
AU  - Balaban N
LA  - eng
GR  - R21 AI054858/AI/NIAID NIH HHS/United States
GR  - R21-AI054858-01/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Agr protein, Staphylococcus aureus)
RN  - 0 (Bacterial Proteins)
RN  - 0 (Phosphoproteins)
RN  - 0 (SAR protein, Lycopersicon esculentum)
RN  - 0 (TRAP protein, Staphylococcus)
RN  - 0 (Trans-Activators)
RN  - EC 2.7.7.6 (RNA Polymerase III)
RN  - EC 3.6.5.2 (Monomeric GTP-Binding Proteins)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing
MH  - Bacterial Proteins/genetics/*metabolism/physiology
MH  - Biofilms/growth & development
MH  - Gene Expression Profiling
MH  - *Gene Expression Regulation, Bacterial
MH  - Monomeric GTP-Binding Proteins/metabolism
MH  - Oligonucleotide Array Sequence Analysis
MH  - Phosphoproteins/genetics/*metabolism/physiology
MH  - Phosphorylation
MH  - RNA Polymerase III/metabolism
MH  - Staphylococcus aureus/*genetics/*pathogenicity
MH  - Trans-Activators/metabolism
MH  - Transcription, Genetic
MH  - Virulence/genetics
PMC - PMC1230921
EDAT- 2005/09/24 09:00
MHDA- 2005/12/13 09:00
PMCR- 2005/10/01
CRDT- 2005/09/24 09:00
PHST- 2005/09/24 09:00 [pubmed]
PHST- 2005/12/13 09:00 [medline]
PHST- 2005/09/24 09:00 [entrez]
PHST- 2005/10/01 00:00 [pmc-release]
AID - 73/10/6220 [pii]
AID - 0436-05 [pii]
AID - 10.1128/IAI.73.10.6220-6228.2005 [doi]
PST - ppublish
SO  - Infect Immun. 2005 Oct;73(10):6220-8. doi: 10.1128/IAI.73.10.6220-6228.2005.