PMID- 16178359
OWN - NLM
STAT- MEDLINE
DCOM- 20051028
LR  - 20190818
IS  - 0031-1820 (Print)
IS  - 0031-1820 (Linking)
VI  - 131
IP  - Pt 3
DP  - 2005 Sep
TI  - Tumor necrosis factor receptor-mediated apoptosis in Trichinella 
      spiralis-infected muscle cells.
PG  - 373-81
AB  - In order to reveal the mechanisms underlying nurse cell formation during 
      Trichinella spiralis infection, the expression of the factors of tumor necrosis 
      factor-alpha (TNF-alpha)/TNF receptor 1 (TNFR-1) signalling pathway mediating 
      apoptosis was investigated. The analysed factors included TNF-alpha, TNFR-1, TNF 
      receptor-associated death-domain (TRADD), caspase 3, caspase 8, TNF receptor 
      associated factor-2 (TRAF2) and receptor interactive protein (RIP), all of which 
      are involved in the TNF-alpha/TNFR-1 signalling pathway-mediated apoptosis. The 
      quantitative RT-PCR indicated that the infected muscle tissues up-regulate the 
      expression of pro-apoptosis genes (TNF-alpha, TNFR-1 and TRADD, caspase 3 and 
      caspase 8), and anti-apoptosis genes (TRAF2 and RIP) at the beginning of cyst 
      formation. The expression returned to the normal level after cyst formation. The 
      quantitative RT-PCR analysis of mRNA from tissue samples isolated by laser 
      capture micro-dissection confirmed that the up-regulation of these genes was 
      restricted in infected muscle cells, was not in the inflammation cells around 
      infected muscle cells nor in normal muscle cells. The in situ localization study 
      of proapoptosis (TRADD, caspase 3) and anti-apoptosis gene products (TRAF2) 
      indicated that these were expressed in the basophilic cytoplasm (infected muscle 
      cell origin) of the nurse cells. Thus the present study suggests that the 
      TNF-alpha/ TNFR-1 signalling pathway is involved in nurse cell formation.
FAU - Wu, Z
AU  - Wu Z
AD  - Department of Parasitology, Gifu University Graduate School of Medicine, Yanagito 
      1-1, Gifu 501-1194, Japan.
FAU - Nagano, I
AU  - Nagano I
FAU - Boonmars, T
AU  - Boonmars T
FAU - Takahashi, Y
AU  - Takahashi Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Parasitology
JT  - Parasitology
JID - 0401121
RN  - 0 (Oligopeptides)
RN  - 0 (RNAIII inhibiting peptide)
RN  - 0 (Receptors, Tumor Necrosis Factor)
RN  - 0 (TNF Receptor-Associated Factor 2)
RN  - 0 (Tumor Necrosis Factor Receptor-Associated Peptides and Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 3.4.22.- (Casp3 protein, mouse)
RN  - EC 3.4.22.- (Casp8 protein, mouse)
RN  - EC 3.4.22.- (Caspase 3)
RN  - EC 3.4.22.- (Caspase 8)
RN  - EC 3.4.22.- (Caspases)
SB  - IM
MH  - Animals
MH  - Apoptosis/*physiology
MH  - Caspase 3
MH  - Caspase 8
MH  - Caspases/metabolism
MH  - Gene Expression Regulation
MH  - Mice
MH  - Mice, Nude
MH  - Muscle, Skeletal/metabolism/parasitology/*pathology
MH  - Oligopeptides/metabolism
MH  - Receptors, Tumor Necrosis Factor/*physiology
MH  - Signal Transduction
MH  - TNF Receptor-Associated Factor 2/metabolism
MH  - *Trichinella spiralis
MH  - Trichinellosis/*pathology/physiopathology
MH  - Tumor Necrosis Factor Receptor-Associated Peptides and Proteins/metabolism
MH  - Tumor Necrosis Factor-alpha/physiology
EDAT- 2005/09/24 09:00
MHDA- 2005/10/29 09:00
CRDT- 2005/09/24 09:00
PHST- 2005/09/24 09:00 [pubmed]
PHST- 2005/10/29 09:00 [medline]
PHST- 2005/09/24 09:00 [entrez]
AID - 10.1017/s0031182005007663 [doi]
PST - ppublish
SO  - Parasitology. 2005 Sep;131(Pt 3):373-81. doi: 10.1017/s0031182005007663.